[ccp4bb] Beamline manager post available, SOLEIL PROXIMA 1.

2014-10-10 Thread THOMPSON Andrew
Hello everyone, Just to draw your attention to this opening for a beamline manager on the PROXIMA 1 beamline at the SOLEIL synchrotron. Gif sur Yvette, France. Anyone interested should look at the job description on the SOLEIL web pages (http://www.synchrotron-soleil.fr/portal/page/portal/Soleil/

Re: [ccp4bb] Refinement of Iron-sulphur clusters

2014-10-10 Thread Robbie Joosten
Dear Stephen, The dictionary is very specific about atom names. If you have them swapped (as many PDB entries do). The angle and chiral volume restraints will wreck your cluster. You also need to make sure to provide LINK records to attach the cluster to the surrounding cysteines. HTH, Robbie

[ccp4bb] Refinement of Iron-sulphur clusters

2014-10-10 Thread
Dear CCP4 BBers, I am currently refining a model of a protein containing three iron sulphur clusters using Refmac (v5.8.0078) and am getting some unusual results. One of the clusters (3fe 4s) seems to behave resonably and the refined electron density looks very nice. The atoms in the others c

Re: [ccp4bb] Biolayer Interferometry

2014-10-10 Thread Cheng Yang
Sent via the Samsung GALAXY S®4 Active™, an AT&T 4G LTE smartphone Original message From: Alfredo Torres Date:2014/10/10 10:08 (GMT-06:00) To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Biolayer Interferometry Dear all, Sorry for the non-CCP4 question. We are evaluating the cu

Re: [ccp4bb] Biolayer Interferometry

2014-10-10 Thread Jurgen Bosch
Whatever you decide to do, it is good practice to confirm your “hit” with an orthogonal method. Interactions in both directions with the same piece of equipment is a start but that in my eyes does not count as another method. If you are trying to decide between the Blitz and an Octet I would def

[ccp4bb] Biolayer Interferometry

2014-10-10 Thread Alfredo Torres
Dear all, Sorry for the non-CCP4 question. We are evaluating the current techniques to measure target-ligand interactions, and we would very much like to know the experience that the members of this community have about the BLItz/Octet system from ForteBio, in particular the rate of false pos

Re: [ccp4bb] coot: protein sequence number too large to be displayed

2014-10-10 Thread Yong Tang
Thanks Tim for the input - so far you are the only person to response so I assume there is not a more straightforward solution to it - like changing a parameter in .coot to allow display of a larger number of residues. Changing and re-changing the residue numbers should work fine for an isolated st

Re: [ccp4bb] Classification of Ig-like domains

2014-10-10 Thread Steiner, Roberto
Dear Thomas There are several Ig-like domains that do not contain S-S bridges . I am not aware of a software tool that does what you'd like. However, two key papers on the Ig-like fold (old but still very relevant) that might be useful to you are: (1) Bork, Holm & Sander - The Immunoglobulin