[ccp4bb] Phaser question, twinning, DIALS, suggestions welcome

Dear BB, or in particular Phaser developers :-) This must be part of British humor right (or was that the Canadian influence Randy) ? eLLG indicates that placement of ensemble "ensemble_1" will be straightforward The data are sufficient to exceed the eLLG target The search space is finite 14

[ccp4bb] Assistant Professor position at the University of Massachusetts Amherst

The Biochemistry & Molecular Biology Department at UMass Amherst is looking for a tenure-track investigator. Please see the advertisement below. Thanks, Scott Scott C. Garman, Ph.D. Associate Professor Department of Biochemistry and Molecular Biology University of Massachusetts Amherst 1021K Le

Re: [ccp4bb] Fedora Core 20 issues.

Hi Dave, the easiest way to install the proprietary Nvidia drivers on Fedora is to use the RPM Fusion repositories. The installation instructions for RPM Fusion are at http://rpmfusion.org/Configuration. Once the configuration files are installed you can install the Nvidia driver as root on th

Re: [ccp4bb] Fedora Core 20 issues.

Thanks for all the suggestions. I think my issue is because I have a QuadroFX1400 video card and I don’t have proper drivers - the generic ones just won’t do it. I have tried and failed miserably to install the proprietary drivers from NVidea, so I’m not going to worry about it. I only use ch

Re: [ccp4bb] Space group numbers

Hi all, Thanks for your help. CORRECT.LP includes precisely the information I was after. Also Ian Tickle’s article on http://www.ccp4.ac.uk/html/alternate_origins.html is very helpful. Simon

Re: [ccp4bb] Space group numbers

Be careful: the International Tables space group number may be ambiguous. For example sg number 18 may refer to P 21 21 2 or its permuted settings P 21 2 21 or P 2 21 21, if you follow the "proper" IUCr convention that primitive orthorhombic space groups have a wrote: > Dear ccp4bb, > > Could

Re: [ccp4bb] Space group numbers

Look at $CLIBD/syminfo.lib All listed by number as well as other flags.. Eleanor On 30 September 2014 13:17, Philip Kiser wrote: > From XDS: > ** LATTICE SYMMETRY IMPLICATED BY SPACE GROUP SYMMETRY ** > > BRAVAIS-POSSIBLE SPACE-GROUPS FOR PROTEIN CRYSTALS > TYPE

Re: [ccp4bb] Space group numbers

>From XDS: ** LATTICE SYMMETRY IMPLICATED BY SPACE GROUP SYMMETRY ** BRAVAIS-POSSIBLE SPACE-GROUPS FOR PROTEIN CRYSTALS TYPE [SPACE GROUP NUMBER,SYMBOL] aP [1,P1] mP [3,P2] [4,P2(1)] mC,mI[5,C2] oP [16,P222] [17,P222(1)] [18,P2(1)

[ccp4bb] Space group numbers

Dear ccp4bb, Could someone either provide, or point me to, a list of space-groups relevant to protein crystallography just by space group number? I can find lots of tables that list them by crystal system, lattice etc. but no simple list of numbers. Thanks, Simon

Re: [ccp4bb] Protein crosslinking

Dear Careina, First: make sure your solution is completely free of competing primary amines, e.g. tris buffer. Phosphate buffers or HEPES should be used; if you need buffer exchange, do it by gel filtration or extensive dialysis (2-3 changes), not concentration-dilution. Second: Try to use BS3,

Re: [ccp4bb] Fedora Core 20 issues.

I take it you have the 32bit libraries installed. They are not normally installed by default. Then you need to make sure the app is linked with these 32bit libraries. I suspect that you may have linked to the 64-bit libraries. Adam > Hi all, > > So, I know this probably isn’t the right plac

Re: [ccp4bb] Fedora Core 20 issues

FWIW for CCP4 we'd recommend 64-bit Linux. Our 64-bit Linux version is downloaded now 6x more often than 32-bit one. So it's more tested, and for some datasets pointless and aimless require more than 4GB of memory (which is 32-bit limit). Marcin On Mon, Sep 29, 2014 at 02:05:32PM -0400, David Ro

[ccp4bb] Protein crosslinking

Dear CCP4 members I do not have any good experience with chemical cross linking, it has not worked for me and I am curious how others have got it to work. In my latest attempt I wished to crosslink two monomers with DSS. The protein is exclusively dimeric according to SEC. I incubated the prote

Re: [ccp4bb] frozen pellet insoluble protein

Andreas, you probably know all this, but I only understood quite recently. What happens is that as ice crystals form you get "brine rejection", the same thing that happens in the arctic when sea water freezes. Therefore you can have protein concentrated in pockets of high salt. Fine for some prot

Re: [ccp4bb] frozen pellet insoluble protein

Dear Andreas, maybe the problem is rather rooted in your expression set up. If the protein was soluble in the small scale test, it is not necessarily the case in a fermenter run. The parameters may have changed so strongly that your protein is now expressed mostly insoluble. To my experience, such