Hi Xiaoxiao,
I have the similar problem like yours currently. I can continually get crystals
which is rock-like crystals but the edges are not very clear. I try to break
them with metal needle but surprisingly I could not break them —— a little bit
like a jelly. I haven’t put them on the beam b
Hi Xiao,
I would poke the crystal with a piece of glass fiber or an oocyte needle to
make sure it is not salt. Some protein crystals can be a little tough
(rubber-like), but they react to poking very differently from rocks (salts) and
will eventually shatter. So far I have found this test to be
Hi everyone,
Sorry for an off-topic question.
I got a problem with crystal dissolving. Basically I got crystals of my
protein in various conditions, most conditions contain PEGs but different
salts. These crystals has very similar shape, so it should not be salt.
Now I am trying to dissolve the
Hi Bing,
you can use the latest version of psico, it provides an
"angle_between_helices" function, which in combination with
"plane_orientation" can calculate angles between planes.
Download:
http://pymolwiki.org/index.php/Psico
Example:
fetch 1ubq, async=0
select ring1, 45/CG+CD1+CD2+CE1+CD2+C
Hi Bing -
You don't need a fancy program to get the angle. If plane_wizard gave
you a vector normal to the ring planes, you can use that. Otherwise,
calculate the cross product between two vectors in the plane (e.g. C1-C4
and C2-C5 in a six-membered ring). This vector is normal to the ring p
Coot? Measure angle
I assume you just want that value and not a script to run for multiple
occasions?
Jürgen
..
Jürgen Bosch
Johns Hopkins Bloomberg School of Public Health
Department of Biochemistry & Molecular Biology
Johns Hopkins Malaria Research Institute
615 North Wolfe
Hi everyone,
I want to tell an angel between two aromatic planes which comes from two
different molecules and cross each other.
First, I tried the easier ways. I presume these two aromatic rings are in the
perfect planes, that is why I tried plane_wizard.py and draw_plane_cgo.py which
work ver
Hello everybody
Why sometimes same enzyme of a homologous series from different
organism show different affinity towards type of metal ion for its
activity..Lets say threonine dehydratase from H pylori show maximum
activity with Mg2+ as a cofactor while the same enzyme from Entamoeba
histolytica
