Hi Xiaoxiao, I have the similar problem like yours currently. I can continually get crystals which is rock-like crystals but the edges are not very clear. I try to break them with metal needle but surprisingly I could not break them —— a little bit like a jelly. I haven’t put them on the beam but will try later.
Kind regards, Wenhe On 26 Sep, 2014, at 7:53 am, Xiao Xiao <victor41...@gmail.com> wrote: > Hi everyone, > > Sorry for an off-topic question. > > I got a problem with crystal dissolving. Basically I got crystals of my > protein in various conditions, most conditions contain PEGs but different > salts. These crystals has very similar shape, so it should not be salt. > > Now I am trying to dissolve the crystal to make sure it is my protein, by > SDS-PAGE and N-term sequencing. I washed the crystals in its original > crystallization buffer few times then transfered them into regular buffer > (500mM NaCl, 50mM HEPES 7.5) with or without 10mM DTT, however the crystal > didn't dissolve. > > I then tried to heat it then add SDS loading buffer to run a gel, I did see > very small amount of protein on the gel, at the correct position, but it's > not enough for N-term sequencing. > > Is it normal for a protein crystal? And does anyone have any suggestion for > dissolving such crystals? >
