Dear Partha and Uli,
We haven't tested it on SLES11 and we overlooked this problem.
It will be fixed in updates. For now as a workaround do:
ln -s libgfortran.so.3.0.0 $CCP4/lib/libgfortran.so.3
(hopefully works, I cannot test it now)
Usually the quickest way to have such problems solved is
to
Thank you all for your suggestions, I copied them below
I also found a paper for making Src and Abl catalytic domains Protein
Science Dec 2005
You can increase the formate concentration and it will be a cryoprotectant
on its own. Test increasing amounts until you find the concentrationthat
freezed clear as glass and the transfer your crystal to this. We did this
with sodium formate at 7 M quite easily.
On Tue, Dec 17, 2013 at 2:59 AM,
I find that odd because the first structure I ever worked on was a DNA
oligonucleotide grown in 0.2 M magnesium formate and it did not require
cryoprotection at all when flash cooled directly in the cryostream. I
learned from Ned Seeman's group a long while back that Mg2+ itself
worked reasonab
Dear Chang,
Some CCP4 progs can be used with a multi-core machine,
using OpenMP threads (including refmac it would appear). You will
need to compile the code from source rather than taking the binary
versions, which is much less convenient.
Even if the CCP4 apps are not parallel thems
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Dear Chang,
some time back people would benefit from the computational time (or
e.g. data collection time) to get a coffee, lean back and think about
what was going on - this was often very efficient and not rarely more
effective than the false percep
Dear Tim
Yes, it is just my impression. I don't know how to test it. As now most of
computers have multi-core CPU, I really hope CCP4 programs can be paralleled.
Thank you so much for answering my questions.
All my best
Chang
2013/12/17 Tim Gruene
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Yes - mlphare did, but so does Phaser_ep
E
On 16 December 2013 18:46, Bosch, Juergen wrote:
> Didn't mlphare use to print those values in the log file ?
> Jürgen
>
> On Dec 15, 2013, at 4:29 PM, David Schuller wrote:
>
> I have some SIRAS data of a known structure. I want to get the
> isomorphou
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Dear Chang,
Without being aware of the various means to parallelise a computer
program I would be surprised if refmac5 was parallelised for MacOSX
but not for Linux, although I am sure Garib would correct me if I was
wrong. Maybe you only have the imp
Thanks so much. XDS program is a very good example for multi-core cpu
processing. I love it. I also have a macbook. Though a notebook, it often
runs CCP4 program more quickly than on desktop computer. The programs, for
example refmac5, seem to use multi-core on OSX and single core on linux. Is
that
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Dear Chang,
this is hardly a matter of configuration but a question of programming
(meaning you cannot change it unless you modify the source code and
recompile). You can run a program of which you know it is
parallelised, e.g. XDS, shelxl, shelxd, an
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Dear Junyu,
You can test cryo conditions conveniently in the absence of your
crystals if you have access to e.g. an inhouse machine. Prepare your
solutions with the usual suspects (PEG400; PEG400+glycerol;
butanediol; Na Malonate etc. pp.) at various
Dear Chang,
As far as I know, most CCP4 programs are single-CPU and would need
extensive changes to make them patallel. Examples of
multiple-CPU programs are XDS, SHELXD, SHELXL and (in part) PHASER.
Best wishes, George
On 12/17/2013 06:51 AM, Chang Qing wrote:
Dear all
I just installed CCP
The kinase domain of FAK can be made constitutively active by a mutation
and expressed in E. coli. It's called "Super FAK Kinase". But i am afraid
it is rather specific. If you don't get a better lead I can send you the
sequence and a reference.
Gloria Borgstahl wrote:
Does anyone know of a good
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