Hi Mahesh,
If you have made these screen shots using pymol, then you really need to be
careful before you jump to conclude anything from these secondary
structural changes (Matt already mentioned it). Assign secondary structures
using DSSP or see the secondary structure restraints in the structure
Dear Mahesh -
I can't comment on the energy changes for the protein, but I can say
that the changes you've highlighted are not really due to alterations of
the protein, but rather inconsistency in assignment of secondary
structure. If you look closely at the areas you've highlighted, you
wil
Dear Colleagues,
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presentation in Rigaku Crystallization Webinars on November 12th, 2013, 8:00am
Pacific Standard Time. The webinar is free to attend and all are welcome. You
can register it through our web page, http://www.
Dear Jan,
There are a few things a would do in this case. The first is to check the
processing to make sure the space group is really C2 and, although unlikely,
not some other space group.
The second thing would be to try to place the first component. From your email
it is not clear to me whe
Dear all,
I have a question regarding Molecular Replacement using low sequence
identity templates.
I have a 2.7 Angstrom dataset of a heterodimeric protein-protein
complex (space group C 1 2 1, no twinning detected using xtriage). For
the first component homologs are available, but for the
Dear Stefan,
The picture you give of your crystals is indeed quite messy. The first thing I
would do is to sort all the information at hand and try to see what could be
going on. Based on the (limited) information you give, I have to following
comments:
1) Autoindexing suggests a tetrag
.and there is always the twilight collection and the gems shown in the
associated paper:
http://www.ruppweb.org/twilight/default.htm
Best, BR
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
Alessandro Nascimento
Sent: Donnerstag, 17. Oktober 2013 23:22
To: CCP4BB@J
