Hi Boaz,
In fact, we have a different crystal form. Well, sort of as it is
related to the first form (a=69.1 c=216.7A P6122 vs. 66.6 103.448
P6222). Obviously the second form has only one molecule/ASU. The
interfaces remain the same with the exception that there is no
problematic inconsistency
Hi,
> I am wondering if I can fix a set of selected atoms during simulated
> annealing in phenix.refine?
no.
Pavel
P.S.: There is Phenix mailing list for Phenix-specific questions
Hi All,
I am wondering if I can fix a set of selected atoms during simulated annealing
in phenix.refine?
I only found the option in xyz refinement, but not in annnealing.
Thank you!
Hi Karolina,
I would suggest you do SAXS. You can compare the
scattering profile you get from your protein in solution to the calculated
profiles of the three possible dimers (or other oligomers) you have in the
crystal structure. This should give you a definitive answer. See for
example this p
Hi Karolina,
You have an interesting case in hand but which is not uncommon. From your description it sounds as if option 2 is more likely to represent the biological dimer but as you noted, it's not certain. Best would be to obtain another crystal form (easily said,
I know, not easily done).
Hi all,
I'm working with a protein that appears to be a dimer in solution, on
SEC in runs as 24 kDa, while the actual mass of a dimer is 30. And I am
trying to figure out which dimer is the biological one (it is a
regulatory protein but details are uknown). The crystal structure gives
me a few
On Jul 13, 2013, at 5:36 PM, Ian Tickle wrote:
> On 8 July 2013 18:29, Douglas Theobald wrote:
>
> > > Photons only have a Poisson distribution when you can count them:
> > > QM says it meaningless to talk about something you can't observe.
> >
> > Aw, come on --- QM is a theory, it says no s
Dear all,
I would like to thank everyone for being so kind and sparing their time to
respond. Just to summarise from all the responses that I got that may help
someone else:
MBP on its own is monomeric and tends to 'magically' help pretty much every
protein you throw at it to become soluble. In m
Thurs. July 25th, 2013
EBI
also an interesting article on the subject of women in science then and
today
http://www.guardian.co.uk/technology/2013/jul/25/rosalind-franklin-google-doodle
Miri Hirshberg, PDBe
On Thu, 25 Jul 2013, David Schuller wrote:
http://news.cnet.com/8301-1023_3-5759541
http://news.cnet.com/8301-1023_3-57595418-93/google-doodle-honors-birthday-of-biophysicist-rosalind-franklin/
Google doodle honors birthday of biophysicist Rosalind Franklin
...
--
===
All Things Serve the Beam
=
Many thanks to all of you for useful links and suggestions.
Best regards...
Hena
On Wed, Jul 24, 2013 at 7:51 PM, Bostjan Kobe wrote:
> Hena
>
> I agree with the responses so far, but I think It may not be a complete
> waste of time looking at the crystallization conditions for similar
> pro
Posted on behalf of Dr. A. Vale (avale_at_ibmc.up.pt), to whom all
enquiries must be directed.
The Fish Immunology and Vaccinology Group of Instituto de Biologia
Molecular e Celular (Porto, Portugal) is seeking for a Post-Doc to work in
the project “Studies towards identification of structural
VACANCY - (SENIOR) POSTDOCTORAL RESEARCHER IN GENE EXPRESSION FOR
STRUCTURAL GLYCOBIOLOGY, University of York, UK
We wish to appoint a postdoctoral researcher to work on the expression of
mammalian genes, for 3-D structural and chemical mechanistic studies, in
the area of eukaryotic glycobiology i
Dear all,
on behalf of Dr. Dmitri Svergun, I would like to draw your attention to
the following EMBO Global funded course
that we will host together with Prof. Cristiano Luis Pinto de Oliveria
in **São Paulo** in January 2014.
regards
Margret
---
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