My experience with xtals frosting in LN2 either in a dewar, while freezing, or
in pucks, has been because the LN2 was contaminated with ice crystals The fog
you see above your dewar when freezing xtals is frozen water vapor...it will
fall and collect in the LN2 and also deposit on the xtals. De
Dear All,
First of all sorry for bringing the non-ccp4 post. As our CCP4 community is
filled with experts in various fields of structural biology: I'd like to
get some help/suggestions from the membrane proteins expert community.
I'm trying to express my membrane protein in Pichia pastoris (SMD11
Dear All,
I got a Se-crystal diffracted to 3.3A. The protein is predicted to be a
coiled coil. After I got the heavy atom positions from a shelxd based
programs from AutoRickShaw, I run phaser and phenix.autobuild to get a
initial phase shown in the attachment. I fit a "standard helix" into the
d
Dear all,
Currently, I was stuck in a coiled-coil crystal. I have two
Se-derivative crystals in similar crystallization conditions. And the cell
parameters of these two are list below :
Crystal A: P21, a=69,b=43,c=135, beta=100.7
Crystal B: P21, a=29,b=230,c=42, beta=92.2
Assuming the crysta
I stumbled across this interesting abstract today, and though I'd rekindle the
perennial data storage debate on ccp4bb.
Apparently these researchers have figured out a way to store 360TB of data on a
"disc" (not sure of the actual dimensions). The memory crystal should have a
thermal stability
At our last synchrotron trip, the beamline staff suggested that the problem
was due to moisture accumulation in the dry shipper. They recommended
storing them inverted (for a few weeks, if I recall), and/or putting a
supply of dry air in the dewer. Haven't tried it yet!
Nat
On Thu, Jul 11, 2013
Hi All,
We found if the crystals are storaged in pucks for 3-4 days in shipping dewar
(with liquid nitrogen), they are almost frosted.
Although I can wash them with liquid nitrogen, but it's not convenient to do
that for each crystals.
I doubt it's because the humid air in North West America.
Doe
Dear all,
In a bit more than a year from now, we will be running the Gordon Research
Conference on Diffraction Methods in Structural Biology,
this time preceded for the first time by a two-day Gordon Research Seminar,
exclusively for young scientists and a few mentors.
http://www.grc.org/progr
PS In fact, the protein I mentioned, was not his-tagged, and first purification
step I used (after streptomycin sulphate precipitation of DNA and dialysis) was
on DEAE-sepharose at pH 8. In this, the protein surprisingly bound, I would
guess via bound nucleic acid contaminants, so in effect the
Hola Careina,
filtering won't help much against aggregation, it just removes the larger
aggregates, but the protein may still form small aggregates that pass the
filter.
and remember the pI from the sequence is only an estimation and may or may not
be close to the real pI, which can only be me
Dear All,
one more position has become available in our lab.
A Research Associate positions have become available in the laboratory for
crystallisation and crystallographic studies on mammalian transporter in the
Membrane Protein Crystallography Laboratory at the Research Complex in Harwell
(RC
Dear ccp4 bulletin board
Sorry for off topic question. I'm working with a protein with pI 9.5 and yet it
will not bind to a cm sepharose column when equilibrated at pH 6.5. I have
removed all salt from the buffer but it still will not bind. I wonder if anyone
has suggestions as to why this coul
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