[ccp4bb] Glutathione rsin

Hi, I would like to ask the bb faithful for their experience with the glutathione affinity resins. We have been using so far the Glutathione Sepharose fast flow from GE but the price is getting steeper. We found Glutathione Superflow resin from Clontech to be significantly less expensive and Glu

[ccp4bb] Re : Re: [ccp4bb] visualizing protein-protein interface

Google 2D-GraLab. Very good at showing detailed interface contacts along protein sequences. To my knowledge, there is no way to include a non protein ligand. But the source code is available on request and if you succeed to modify the program, please let us know. HTH, Nadir Mrabet Pr. Nad

Re: [ccp4bb] visualizing protein-protein interface

This is the kind of things MG was designed to do -- Eugene On 4 Jun 2013, at 15:18, Tobias Beck wrote: Dear all, I would like to visualize the interface of a protein-protein complex. The complex formation is modulated by a ligand that upon binding alters the overall structure of one protein, w

[ccp4bb] Postdoctoral position at University of Leipzig, Germany

A full-time postdoctoral position is available at the Institute of Bioanalytical Chemistry, University of Leipzig, Germany. We are seeking an independent and highly motivated postdoctoral researcher for protein design and structural studies on redox enzymes in biocatalytic applications, includ

[ccp4bb] Helix forming proteins crystallizing as rings

Dear all, can anyone provide me with examples for proteins that form a helical oligomer under physiological conditions and flat rings (basically a helix with a pitch of 0) in the crystal? I only know examples of the other way around. Thank you in advance. Joern

[ccp4bb] protein tags

Dear All, We are looking to protein tags (of size not exceeding 10 kDa) as markers to label our protein complex of size ~50 kDa. Although I have searched for several possible candidates mainly mini-proteins, most of them are not what we want to have. The features that we are looking in the tag

Re: [ccp4bb] visualizing protein-protein interface

Hi Tobias, this may or may not work. calculate a map for both proteins, normalize them, put them on an identical cell and subtract one map from another. The program that can do this is called mapman *gosh* a non-CCP4 thing. Good luck, Jürgen On Jun 4, 2013, at 10:18 AM, Tobias Beck wrote: Dear

[ccp4bb] visualizing protein-protein interface

Dear all, I would like to visualize the interface of a protein-protein complex. The complex formation is modulated by a ligand that upon binding alters the overall structure of one protein, which then in turn forms the complex with the second protein (structure of the complex is known). Ligand bin

[ccp4bb] Feedback about Dionex HPLC units for protein purification purposes

Hello all, Sorry for the off-topic query but does anyone here have any feedback to share, positive or negative, about using Dionex HPLC instruments for preparative protein purification, i.e. using these in lieu of AKTA units ? Any help will be much appreciated. Best, Anirban

[ccp4bb] Technician position available at the SLS

http://www.psi.ch/pa/offenestellen/0595-1 We are looking for a Technician Protein Crystallization Facility and Crystallography Beamlines Your tasks You will be responsible for helping with the everyday operation and maintenance of the crystallization and liquid handling robots within the macrom