Re: [ccp4bb] Difficult data

Hi Everybody, Thanks so much for all the help! I suppose I should give more info. Marco, the data is twinned - I forgot about that in my initial posting. I can't remember the stats, but it is not a perfect twin. I thought that Phaser accounted for twinning, and I haven't got a solution good eno

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Re: [ccp4bb] Difficult data

Yes- this has happened to us so often recently that I've taken to checking every new crystal form against the "nearest cell" server before starting heavy atom search. That would account for the poor reproducibility. Yeast glutamate-cysteine ligase (e.g. 3LVV) crystallizes with nearly identical

Re: [ccp4bb] Difficult data

Maybe you crystallized something else? Did you look up that unit cell in the PDB? I am having a few issues with a data set I have been working on recently, and was hoping to get some ideas on how to deal with it, if anyone is in the mood. .

[ccp4bb] Difficult data

Hello, I am having a few issues with a data set I have been working on recently, and was hoping to get some ideas on how to deal with it, if anyone is in the mood. I have been working with a very small bacterocin (about 3 kDa) and set up some crystal trays in hope of getting some high diffracting

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Re: [ccp4bb] Puzzling Structure

Just as a postscript. It has been pointed out to me that the space group was correct in the uploaded PDB and in the mtz reflection file. And there was no editing of this data pre-deposition.  I am sort of surprised how quickly people are to beat up on the authors in such cases since we do not

Re: [ccp4bb] need suggestions

Hi Afshan, You mention: The protein prep is same which was using to get the Hits. So how old is your prep ? Was it stored at 4C or in LN2? Do you know if your protein is stable under the conditions you stored it ? How long after purification did it take you to get those crystals ? How old is

Re: [ccp4bb] need suggestions

Hi Afshan, Have look at http://www.douglas.co.uk/Scaling_Up.htm, Patrick has some general tips on scaling up. Elsewhere on his site you'll find tips for conversion to microbatch, you may want to try that out as well. I assume the screen crystals were not big enough to mount? Flip Op 4/16/20

Re: [ccp4bb] need suggestions

-BEGIN PGP SIGNED MESSAGE- Hash: SHA1 Dear Afshan, - - Can you reproduce the crystals in the Linbro plates with the identical solution used for the screening plate? - - Did you compare the composition of the Linbro plate vs. the screening plate? - - Did you check e.g. the pH of the origin

[ccp4bb] need suggestions

 Dear All, I have encountered one problem to optimization the crystallization condition manually. Actually i got the good shape and even size crystal in a screening plate. The condition are : 20% PEG 6000, 0.1M MES pH 6.0 whereas protein in 10mM Na-acetate pH 5.7 contain 50mM NaCl, 1mM EDTA &5