[ccp4bb] Orientation of the crystal and it importance in data collection

2012-10-26 Thread Vijayakumar.B
Dear CCP4BB users, I have some basic questions in the data collection. Please give me some ideas to get clear in this part. 1)Why orientation of the crystal is importance? 2)If we mounted the crystal in arbitrary, what it leads? 3)How to find out crystal misseting angels in the

[ccp4bb] Postdoc crystallographer position in Houston

2012-10-26 Thread Spudich, John L
Description: An NIH-funded postdoctoral position is immediately available to study microbial rhodopsins (channelrhodopsins, other sensory rhodopsins, and ion transporters) by x-ray crystallography and biochemical/biophysical methods. The position requires a recent Ph.D. in one of the natural sci

Re: [ccp4bb] adding some user-defined graphical objects in a PDB file (and displaying them)

2012-10-26 Thread Pete Meyer
Just out of curiosity, why use PDB format instead of converting PDB into a format readable by a more general 3d graphics program and combining with your cube/sphere/line segment there? Pete Francois Berenger wrote: Hello, For some new project, I'd like to be able to generate things and store

[ccp4bb] Postdoctoral position at the SGC, University of Oxford

2012-10-26 Thread Opher Gileadi
We are seeking a postdoctoral scientist to join the group of Opher Gileadi, studying DNA repair and the structural basis of human genetic disease. This position offers a unique opportunity for a structural biologist. You will drive several projects from gene to structure, aimed at understanding

Re: [ccp4bb] Compounded problems for molecular replacement

2012-10-26 Thread Phil Jeffrey
Hello Jose, Depending on what data integration program you used, trying XDS may help you out a little with spot overlap. Example #3 in my rather out-of-date page: http://xray0.princeton.edu/~phil/Facility/Guides/MolecularReplacement.html illustrates how you could find 8 domains, especially if

Re: [ccp4bb] Compounded problems for molecular replacement

2012-10-26 Thread Roger Rowlett
I had a similar issue (8 chains in a large ASU, 2.4 A resolution, 29% homology, possibly--and ultimately confirmed--twinned data). Here is how I solved my structure (not pretty!): 1. Phaser was used to find an initial placement for the chains using (what turned out to be) the twin maps. This

[ccp4bb] Position, Research Technician at EMBL Grenoble

2012-10-26 Thread Jose A. Marquez
Dear All A research technician position is currently available in my laboratory at EMBL Grenoble. You will find the details in the link below or at www.embl.org/jobs http://www.embl.de/aboutus/jobs/searchjobs/index.php?newlang=1&newms=sr&searchregion=670

Re: [ccp4bb] Compounded problems for molecular replacement

2012-10-26 Thread Bosch, Juergen
We had recently a similar case. Indexed in P2x2x2x but truly was P21 with variable amount of twin fraction depending on the dataset & beamline between 9% and 40%. We were able to solve it using phenix.ensembler using all available known structures plus our various homology models. We still have n

[ccp4bb] Compounded problems for molecular replacement

2012-10-26 Thread Seijo, Jose A. Cuesta
Hi all, I am dealing with a molecular replacement problem for a 60KDa protein composed of 2 rigid domains joined by a flexible linker which can move relative to each other. Sequence identity for my best model is 46% evenly spread, so in principle this should be a tractable problem. Then the

Re: [ccp4bb] Resuspension of bacterial cell pellets

2012-10-26 Thread Roessler, Christian
This is the heating information found on BioSpec's website: Like many mechanical cell disruption techniques, the Beadbeating process generates heat. In general for every minute of beadbeating there is a 10 deg rise in sample temperature. Should that be a problem, the ice-water jacket included w

[ccp4bb] Off topic: Meeting on X-ray imaging of whole, unstained biological cells

2012-10-26 Thread elizabeth . duke
Apologies about the off-topic nature of this posting. Some of the wider structural biology community may be interested in this meeting taking place at Diamond Light Source on December 5/6 2012. Diamond Light Source are hosting an Instruct funded 2-day meeting on cryo soft X-ray tomography of bi

[ccp4bb] Postdoc position single-particle cryoEM at EMBL

2012-10-26 Thread Carsten Sachse
Dear all, The Sachse group at EMBL offers a postdoc position in structural biology. Single-particle cryo-EM of cytoplasmic inclusion bodies: http://ig14.i-grasp.com/fe/tpl_embl01.asp?s=bkMjPUrEcTFkHhTcz&jobid=49883,5852540212&key=51280717&c=726187025436&pagestamp=sefjdpnrpgtsnkotgc. Application