The multidrug efflux transporter AcrB could be suitable including
crystallization (both recombinant with His-tag and endogenous). Extracts,
solubilizes, purifies and crystallizes in DDM, so it is fairly inexpensive to
repeat in bulk compared to other detergents. Assays might be a bit complicated
Hi,
Just for info if you were to use the LCP method (a course by itself), check
this about OmpF ("membrane lysozyme"):
http://www.sciencedirect.com/science/article/pii/S1047847712000834
bR protein sometimes takes weeks to give crystals and people prefer the
dark (depends on the conf state).. but go
Two additional suggestions:
1. Photosynthetic Reaction Center from *R. sphaeroides* might be up your
alley. Similar to bacteriorhodopsin, no need for a GFP tag on this one as
it's colored. See the following publications:
Wallace et al. "Monoolein Lipid phases as incorporation and inrichment
mat
Wouldn't bacteriorhodopsin be a good choice, especially since it's colored
and easy to express? Seems pretty expensive for a class, though, with all
the detergents involved...
JPK
On Tue, Sep 11, 2012 at 4:18 PM, Ho Leung Ng wrote:
> Hello,
>
> I am developing an undergraduate biochemistr
Hello,
I am developing an undergraduate biochemistry lab class and
would like to incorporate experiments with membrane proteins. Does
anyone have suggestions on membrane proteins that are relatively easy
to express, purify, and assay? Bonus points for crystallizable! At the
moment, my leadin
Dear crystallography community,
I wanted to make the community aware of a new tenure-track faculty position
at Brown University in structural biology. Details about the position are
provided below.
*Structural Biology Faculty Position, Assistant Professor*
The Department of Molecular Biology, Ce
Assistant Professor – Structural Biology. The Department of Chemistry
and Biochemistry
(http://chem.ou.edu) at the University of Oklahoma (http://www.ou.edu)
invites applications for
a tenure-track faculty position at the rank of Assistant Professor
beginning August 16, 2013. We
are especia
There are 2 issues raised here and the answers are:
1) It is absolutely normal that the same protein crystallizes in two or
more space groups. It may even happen under the same crystallization
conditions, due to the randomness of nucleation. It happens more often
when crystallization conditions ar
You may want to check that the distance, 2-theta and wavelength are correct.
Kris F. Tesh, Ph. D.
Department of Biology and Biochemistry
University of Houston
From: "herman.schreu...@sanofi.com"
To: CCP4BB@JISCMAIL.AC.UK
Sent: Tue, September 11, 2012 1:29:26 A
:-) yes it did!
problem solved!!
Thanks a lot Claus
Rosa
On 09/11/2012 05:19 PM, Claus Flensburg wrote:
Dear Rosa,
On Tue, Sep 11, 2012 at 04:51:44PM +0200, Rosa Grenha wrote:
Dear all
I have been fighting to solve this problem in running buster and
would appreciate any advice on the subject
Dear Rosa,
On Tue, Sep 11, 2012 at 04:51:44PM +0200, Rosa Grenha wrote:
> Dear all
>
> I have been fighting to solve this problem in running buster and
> would appreciate any advice on the subject (errors shown below).
>
> I have installed ccp4-6.3.0 and buster gives me now an error related
> to
Dear all
I have been fighting to solve this problem in running buster and would
appreciate any advice on the subject (errors shown below).
I have installed ccp4-6.3.0 and buster gives me now an error related to
environ.def
It runs without any problems with the previous version of ccp4 and t
Dear All,
This is a reminder of the joint EMBL-CCP4 training course "European School for
Macromolecular Crystallography (ESMAX)", which will take place at the EMBL
Hamburg Outstation, the DESY synchrotron site during the period:
Monday November 19th to Monday November 26th, 2012.
The ESMAX-20
On Sep 6, 2012, at 9:00 AM, eugene.krissi...@stfc.ac.uk wrote:
> If this does not work for you for any reason, please (re-)read update manual
> for details. If that does not help as well, please write to us.
Dear Eugene:
Will there be a way to get the corresponding changes to the source-code?
Just a reminder that the closing date (30th September) for registering for this
BioSTRUCT-X funded meeting is fast approaching. The meeting is aimed at
synchrotron users wishing to get the most out of multi-circle goniometers /
pixel array detectors.
Workshop on Advanced Data Collection with
EXCELLENT !!!
Dr Felix Frolow
Professor of Structural Biology and Biotechnology, Department of Molecular
Microbiology and Biotechnology
Tel Aviv University 69978, Israel
Acta Crystallographica F, co-editor
e-mail: mbfro...@post.tau.ac.il
Tel: ++972-3640-8723
Fax: ++972-3640-9407
Cell
Dear Sudipta,
Use Phenix eLBOW. Give the input SMILES string and get the pdb and cif
restraint files as output. Also you can use
http://cactus.nci.nih.gov/translate/ to first get the pdb coordinate file from
the SMILES string and then run Phenix eLBOW to get the cif restraints.
Best regards,
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