Re: [ccp4bb] mosaicity and SAD

Dear Supratim, In my experience, as long as you do not run into an overlap problem, a large mosaicity is not a problem. If the statistics look good, you can safely use the data. However, if the completeness of the data is much lower than what was predicted, many spots may have been thrown out bec

Re: [ccp4bb] mosaicity and SAD

Hi friends what is the maximum mosaicity value to work with. When i am doing Heavy metal replacement my mosaicity value is around 1 - 1.2 after processing through HKL. I don't know is it acceptable data. I have used mercuric chloride for my protein and it has shown good results .However in case

[ccp4bb] mosaicity and SAD

Hi friends what is the maximum mosaicity value to work with. When i am doing Heavy metal replacement my mosaicity value is around 1 - 1.2 after processing through HKL. I don't know is it acceptable data. I have used mercuric chloride for my protein and it has shown good results .However in case of

[ccp4bb] Postdoctoral postion

Hi, A post-doctoral opening in protein crystallography in the laboratory of Prof. Chris Dealwis at the Cleveland Center for Membrane and Structural biology. The project involves structure determination of protein-protein complexes and protein drug interactions. We are seeking for an individual tha

Re: [ccp4bb] Jelly body refinement?

Hi Rob, thank you, your comments helped a lot. From the Refmac5 paper I did not get the fact that d is set to d_current after each step. In that case you are right, jelly-body corresponds rather to DEN with gamma=1 than to gamma=0. And of course, a very important difference is, as you said,

Re: [ccp4bb] Off topic: Request for a soft copy of a book

Dear all, Thank you for your help and suggestions. Actually I need to consult the book in order to analyze some of our experimental results and I was in a bit hurry. Honestly my intention was not however, to violate the copyright issues but I thought scientific problems demands much than the mere

[ccp4bb] Now Hiring at Microlytic: Application Scientist

*Microlytic is excited to announce the following opportunity: Applications Scientist* * * *Please direct all formal and informal inquiries to j...@microlytic.com* *Description* * * The qualified applicant will possess a Ph.D. in Biochemistry and/or structural biology. Your activities will s

[ccp4bb] MSc in Structural Biology via the web at Birkbeck

The correct URL for the MSc in Structural Molecular Biology via the web at Birkbeck is: http://www.bbk.ac.uk/study/pg/science/TMSBISCL.html. Dr Tracey Barrett, Crystallography, Institute for Structural and Molecular Biology, Birkbeck College, Malet Street, London WC1E 7HX Tel: 020 7

[ccp4bb] REMINDER *CALL FOR PROPOSALS FOR ESRF BEAM TIME WITH ONLINE MICROSPEC*

*CALL FOR PROPOSALS FOR ESRF BEAM TIME WITH ONLINE MICROSPEC*Proposal Deadline *4th September 2012* There will be beam time available at the ESRF for MX data collection with a setup that allows online monitoring of UV/VIS absorbance or fluorescence spectral changes of the crystal during the X-

[ccp4bb] Protein Crystallography course via the web at Birkbeck College

Dear all, registration is currently open for the postgraduate certificate course in Protein Crystallography via the web at Birkbeck that starts on Monday October the 1st. It is for the duration of 1 year during which all aspects of protein crystallography will be covered from the fundame

[ccp4bb] UV microscope

Hi All, May I ask opinion of those who currently have standalone UV microscope whether or not they are happy with their choice? Few that I know are from Formulatrix, JanScientific and Corima. Pros and cons of your instument are greatly appreciated. I'll send the summary to the list. Regards,

Re: [ccp4bb] Off topic: Request for a soft copy of a book

-BEGIN PGP SIGNED MESSAGE- Hash: SHA1 Dear Sudipta, wouldn't distributing such a soft copy ("the unprinted digital document file", wikipedia) be pretty illegal, at least in most countries? If this is the case, your email does not follow this list's etiquette. I appreciate being corrected

Re: [ccp4bb] Off topic: Request for a soft copy of a book

There is a web site used.addall.com that searches through many used booksellers. It appears that there are copies available for under $3.00 (plus postage, obviously). Frances = Bernstein + So

[ccp4bb] High chi square value

Dear All, I am collecting one data set (resolution around 2.4) for a protein SG P21. After collecting few frames (42 frames) the completeness is showing 49%, R merge 11%, mosaicity is 1.08, intensity 9.7. but the chi square value is showing very high, around 3.061. I tried changing error scale

[ccp4bb] Senior computational scientist required for CCP-EM, Harwell, UK (IRC50666)

The CCP-EM project aims to provide computational support for scientists and software developers using electron cryo-microscopy for structural biology, by analogy with similar successful projects in macromolecular crystallography (CCP4) and biological nuclear magnetic resonance spectroscopy (CCPN

Re: [ccp4bb] Plastic and glass plates

Hi Teresa, An additonal disadvantage of using plastic plates would be the polarisation of the plastic. Many users use cross-polarisation to image LCP plates. Flip Op 8/29/2012 21:24, Theresa Hsu schreef: Dear all Is there any pros and cons of using plastic plates for LCP crystallization? T

Re: [ccp4bb] Plastic and glass plates

Also, plastic plates are suitable for in-situ diffraction tests where glass ones cannot be used due to the high absorption of X-rays by glass. Best wishes, Tadeusz From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Jim Fairman Sent: 29 August 2012 21:19 To: CCP4BB@JISCMAIL