Also, plastic plates are suitable for in-situ diffraction tests where glass ones cannot be used due to the high absorption of X-rays by glass.
Best wishes, Tadeusz From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Jim Fairman Sent: 29 August 2012 21:19 To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Plastic and glass plates Theresa, The major advantage of the plastic plates is indeed ease of harvest. However, the plastic plates also tend to have some evaporation issues and eventually dry out after a few months, where as the glass plates basically last forever. On the other hand, protein crystals in LCP tend to form in the first several weeks after an experiment is set up so this usually isn't a problem. Some people prefer to set up initial screening experiments in the glass plates and then optimize and set up "farm" trays using the plastic plates where it's easier to harvest from (and cheaper). I've also set up everything in plastic trays from the start of a project to finish without any problems. If you're looking to perform LCP FRAP experiments, glass is the best way to go. Cheers, Jim On Wed, Aug 29, 2012 at 12:24 PM, Theresa Hsu <theresah...@live.com<mailto:theresah...@live.com>> wrote: Dear all Is there any pros and cons of using plastic plates for LCP crystallization? The glass is clearer but it is very difficult to open. Thank you. -- Jim Fairman, Ph D. Crystal Core Leader I Emerald BioStructures<http://www.emeraldbiostructures.com/> Tel: 206-780-8914 Cell: 240-479-6575 E-mail: fairman....@gmail.com<mailto:fairman....@gmail.com> jfair...@embios.com<mailto:jfair...@embios.com>
