Dear all,
Sorry for non-crystallography related question.
We are trying to express an eukaryotic heme protein in E. coli. We are able to
express it in soluble form. When we use 5-Aminolevulinic Acid, E. coli becomes
brown. However, after cell lysis the soluble protein contains no heme and the
pe
On Jul 13, 2012, at 4:00 PM, Christian Roth wrote:
> I want align a couple or protein structures by secondary structure matching
> to
> one target and want get a kind of aminoacid alignment file e.g. what residue
> fit
> the other, without adjustments due to sequence based alignments.
> I tri
On Sunday, 15 July 2012, Dale Tronrud wrote:
>There are good reasons for maintaining "order" in this human-induced
> numbering scheme. A common operation is to superimpose two molecules
> and calculate the rmsd of the positional differences. This calculation
> is not useful when the Val CG1 a
Dear All,
I am interested in cross-linking of a protein molecule with a complex
(isolated). The protein is expressed recombinantly from E.coli and we are
would like to precisely map the residues involved in interaction. So far,
all other crosslinking methods (targeting amines etc.) have not yielde
On 7/13/2012 1:58 AM, Tim Gruene wrote:
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Dear all,
I am surprised by the discussion about "chiraliy" of an utterly
centrosymmetric molecule. Shouldn't the four Oxygen atoms be at least
from a QM point-of-view to indistinguishable? What reason is ther
