Dear All, I am interested in cross-linking of a protein molecule with a complex (isolated). The protein is expressed recombinantly from E.coli and we are would like to precisely map the residues involved in interaction. So far, all other crosslinking methods (targeting amines etc.) have not yielded any good results. I have read about mapping interaction through non-natural amino acids.
Has someone done it in recent times. Is it possible to suggest some literature, resources or protocol that can be useful. I will be highly grateful. Thanking you all. jan
