[ccp4bb] Sunhats for plants

2012-07-09 Thread Gerard DVD Kleywegt
Hi all, Plants suffer from DNA damage caused by ultraviolet light in the same way that humans do. Unlike us though, they cant put on a sunhat (or move to England) to avoid the suns rays. Read more about how plants sense UV-B light and turn on a suite of genes to protect their DNA against its d

Re: [ccp4bb] MR solution

2012-07-09 Thread Airlie McCoy
Phaser no longer fixes the space group after the first translation function. Phaser carries the space group with each potential solution. If the solution is clear at the end, so will the space group be clear. In practice, we found that the space group was not correct after the first translatio

Re: [ccp4bb] MR solution

2012-07-09 Thread Hermes
If there is such a quirk then it's not one we know about. We would definitely appreciate being sent whatever is needed to reproduce the behaviour. Dimers in orthorhombic space groups can lead to translational NCS, which is handled much better in the latest versions of Phaser. Is it possible Phas

Re: [ccp4bb] SOMoRe

2012-07-09 Thread Airlie McCoy
On Jul 9 2012, James Stroud wrote: On Jul 9, 2012, at 11:02 AM, Tim Gruene wrote: Dear Fulvio, http://lmgtfy.com/?q=somore+molecular+replacement should help you out, especially the but last paragraph of the first link provided. Not Found The requested URL /~djamrog/somore.html was not f

Re: [ccp4bb] MR solution

2012-07-09 Thread David Schuller
Scaling should be the same in P222 vs. P212121. The only difference is the exclusion of systematic absences. You may have run into some quirk of Phaser in the way it handles multiple space groups vs. a single one. On 07/09/12 14:06, Shya Biswas wrote: Hi all, I have a dataset that I scaled i

Re: [ccp4bb] SOMoRe

2012-07-09 Thread Tim Gruene
-BEGIN PGP SIGNED MESSAGE- Hash: SHA1 Hi James, I was actually referring to the email address but just noticed that the domain 'xtal.biochem.wisc.edu' also does not exist anymore. The modification date 2005 is also not very encouraging for finding a working copy of somore... Maybe the TO

[ccp4bb] MR solution

2012-07-09 Thread Shya Biswas
Hi all, I have a dataset that I scaled in p212121 with cell dimension a=28.9 b=67.1 and c=93.5 however I do not get a right MR solution with this. So I went back and scaled it in p222 space group and asked phaser to find the right spacegroup solution for it, this time phaser gave me the right solu

Re: [ccp4bb] SOMoRe

2012-07-09 Thread James Stroud
On Jul 9, 2012, at 11:02 AM, Tim Gruene wrote: > Dear Fulvio, > > http://lmgtfy.com/?q=somore+molecular+replacement > > should help you out, especially the but last paragraph of the first > link provided. Not Found The requested URL /~djamrog/somore.html was not found on this server.

Re: [ccp4bb] SOMoRe

2012-07-09 Thread Tim Gruene
-BEGIN PGP SIGNED MESSAGE- Hash: SHA1 Dear Fulvio, http://lmgtfy.com/?q=somore+molecular+replacement should help you out, especially the but last paragraph of the first link provided. Best, Tim On 07/09/12 17:57, fulvio saccoccia wrote: > Dear ccp4 users, does anyone know where to down

[ccp4bb] SOMoRe

2012-07-09 Thread fulvio saccoccia
Dear ccp4 users, does anyone know where to download SOMoRe for molecular replacement? Thanks in advance Fulvio Saccoccia PhD student Dept. of Biochemical Sciences Sapienza University of Rome Italy

Re: [ccp4bb] Rfactors stuck very high

2012-07-09 Thread Garnett, James A
Dear Garib, I have run the jellybody refinement and whilst it has not reduced the Rfactors I am going to see whether I can see anything usable in the new maps. many thanks james Dr James Garnett Centre for Structural Biology Division of Molecular Biosciences Level 5 Sir Ernst Chain Building S

Re: [ccp4bb] Rfactors stuck very high

2012-07-09 Thread Garib N Murshudov
Dear James It seems that at this stage you may not be able to distinguish between these space groups. As I see they are subgroup of each other (more precisely P1 < C2 < I222 with the cell parameters you have) and it would not be easy to find out the exact space group at this stage. Highest spac

[ccp4bb] 11 PhD positions at the Bijvoet Center in Utrecht

2012-07-09 Thread Bert Janssen
The Bijvoet Center of Utrecht University offers 11 PhD Student Positions in Structural Biology The Bijvoet Center at Utrecht University offers 11 interdisciplinary PhD student projects in structural biology in the Marie Curie Innovative Doctoral Programme (IDP) ManiFold: Manipulating folding, asse