Sure, but many times one's got to live with what one gets, ;-). By the way,
I keep myself interested in bad, ice "ringed" or whatever, images, that
cannot be processed easily and so I try the best I can, as far as I still
cannot produce proteins/crystals in the lab here.
Cheers,
Jorge
On Wednesda
It is better to spent time learning how to collect without ice… :-)
FF
Dr Felix Frolow
Professor of Structural Biology and Biotechnology
Department of Molecular Microbiology
and Biotechnology
Tel Aviv University 69978, Israel
Acta Crystallographica F, co-editor
e-mail: mbfro...@post.tau.ac.il
You may want to have a look at 3ur7 and 3ur8. I have also another example
of a glycohydrolytic enzyme with an N-terminal His-tag sitting in the
active site of a symmetry-related molecule (not deposited yet).
Karolina
On 27/6/2012, "Brad Bennett" wrote:
>I think it was an N-terminal RGS-type H
I thank for the references and the comprehensive discussion from Dr.
Holton. Also, for the reference indicated by Dr. Berry. I think I will get
what I am looking for, now I need to "process" all this information.
Partially answering Dr. Holton, my aim is to have a side guide for
improving
Human leukotriene C4 synthase (PDB accession code: 2UUI) is another
example, illustrating how an N-terminal polyhistidine-tag, in
conjunction with metals, presumably facilitated crystallization.
On Jun 27, 2012, at 12:04 PM, Brad Bennett wrote:
I think it was an N-terminal RGS-type His tag i
Or indeed support surreal structures :-))
(PMID: 11853672 vs PMID: 14749821 and so on)
--
A. Radu Aricescu, PhD
University Research Lecturer
MRC Career Development Award Fellow
University of Oxford
Wellcome Trust Centre for Human Genetics
Division of Struct
Here's another example:
http://www.pdb.org/pdb/explore/explore.do?structureId=2F62
dimer with "His-tag-ears" without His6-tag this would not have been possible.
Jürgen
On Jun 27, 2012, at 12:04 PM, Brad Bennett wrote:
I think it was an N-terminal RGS-type His tag in 3O8Y (human lipoxygenase) tha
I think it was an N-terminal RGS-type His tag in 3O8Y (human lipoxygenase)
that mediated crystal contacts with a symmetry related molecule. As I
recall, this tag composed a B-strand that formed a nice interface with a
"native" B-strand of the symmetry related molecule. Pretty cool...
-Brad
On Wed
A PhD studentship is available in Dundee to work in the laboratory of Bill
Hunter. The project involves the application of crystallographic methods to
support early stage drug discovery targeting bacterial pathogens. A series of
targets are available and the student will be engaged in protein pu
A postdoctoral position is available in the laboratory of Dr. Yongqun Zhu in
Life Sciences Institute, Zhejiang University. Our lab interest is to take
approaches of structural biology to study the pathogen-host interactions and
cellular signaling transduction involved in cancer biology. In addi
With Flp recombinase - DNA complexes, a C-terminal His tag triggered a
different (but sadly not better) crystal form, and the His side chains packed
against the bases at the end of a neighboring DNA duplex.
=
Phoebe A. Rice
Dept. of Biochemistry & Molecular
Most of the comments you will get will be anecdotal in that people will report
the successful results and do not take the time or effort to characterize the
less successful results. This often occurs because the tagged portion of the
protein is most often disordered, even in the best crystals.
Hi,
Two educational softwares which might help you (off course in conjunction
with books and articles) are
1) XrayView
2)SpacegroupViz
Best
Arko
On Wed, Jun 27, 2012 at 6:22 PM, Ed Pozharski wrote:
> Your question is way to broad to be answered in a reasonable
> time/space.
>
> As for books
Your question is way to broad to be answered in a reasonable time/space.
As for books (plenty of options exist beyond these)
There is a 1976 classic
http://www.amazon.com/Protein-Crystallography-Molecular-Biology-Series/dp/0121083500
And of course there is a more recent highly recommended
htt
We have an in-house Agilent (Oxford) system and routinely use data with
CCP4. You will need to run sortmtz, scala (w/constant scale), and truncate
to prep the data properly. This can be done via batch file or GUI.You may
also have to reset/reassign the space group for some space groups due to an
ap
Hi all , thank you for being new member in this group. i also just stated with
my PhD in structural biology at Hamburg University (DESY) . i have no Idea
about crystallography . I just go step by step. so please help me to find my
way for excellence in structural biology. i appreciate your sugg
Just to be clear, Phaser does not require the reflections to be in a
standard asymmetric unit, only that they are unique (as the error message
thrown says).
Airlie
On Jun 27 2012, Eleanor Dodson wrote:
I suspect you didn't request the FreeR assignment on the TRUNCATE
interface? This calls am
I suspect you didn't request the FreeR assignment on the TRUNCATE interface?
This calls amongst other programs, CAD and CAD makes sure that the reflection
list is unique and that it is in a standard asymmetric unit. Most people do it
by default so don't hit these problems...
I suggest you just
Dear Chris,
The script might work technically, but applying the same
transformation matrix 100 times to the output of the previous
iteration is a recipe for disaster. PDB files only store
coordinates to 3 decimal places and your rounding errors will
build up horribly.
Far better to work out t
on behalf of Prof. Michael Sattler:
JOB ADVERTISEMENT:
The CRC 1035 “Conformational Control of Protein Function by conformational
switching“ investigates conformational transitions of proteins and protein
complexes using various biophysical and biochemical techniques. To
com
On 27/06/12 10:03, Froehlich, Chris wrote:
Dear colleagues,
I want to apply a given x-y-z-rotation/transformation matrix on a pdb
file, save the new pdb file and apply the same matrix again on the new
pdb file and repeat this e.g. 100 times, thereby saving all pdb files
(i.e. 01.pdb, 02.pdb,
Dear colleagues,
I want to apply a given x-y-z-rotation/transformation matrix on a pdb file,
save the new pdb file and apply the same matrix again on the new pdb file and
repeat this e.g. 100 times, thereby saving all pdb files (i.e. 01.pdb, 02.pdb,
03.pdb etc.).
With pdbset this is very easy,
Dear Steve,
The mtz file produced by the current version of CrysAlisPro is scaled but
unmerged and requires SCALA to produce the merged data set. The new version of
CrysAlisPro (currently under testing) will generate the merged mtz file as well.
With regards,
Tadeusz
Agilent Technologies
Fro
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