Hi Hongjun,
The 'spherulites' could be DDM crystals. The rods/needles DO NOT seem
like DDM crystals.
A carefully done SDS-PAGE could clarify further. Alternatively, you
could use the sample buffer (no protein) against the same
crystallization reagent to find out.
Veer.
Veer Sandeep Bhatt
Generally speaking it is quite hard to crystallize DDM since it is so soluble
(>20% in water). You most likely have protein crystals (of course containing a
lot of detergent as well) that are just not ordered, presumably because most or
all of the lattice contacts are mediated by detergent and n
Hello All,
Follow up on the problem. With some help from Randy Read, it was
discovered that the sequence file had four chains designated which was
causing the problem. I found out later from the researcher whose
project this is that the structure they were using had 4 copies in the
asy
Alaksa,
1) What rmsd / sigma are you contouring your density at ? i.e. are you down in
the "noise" or are you at a reasonable value for your Fo-Fc map?
2) It looks like some of your side-chains appear to have more than one
conformation - it's fairly easy in Coot to position and model both.
To
Hi,
For me it is detergent crystals or other stuff that is not quite important in
this case. As you showed the resolution is quite low. I suggest you need to
repeat again again again. You may go to PEG200. In our case, it is very
difficult to reproduce membrane protein crystals.
Good luck!
Those don't look like DDM crystals I've seen, but the diffraction pattern
does not look much like protein diffraction either. Which things from the
picture did you shoot--the rods/needles?
Jacob
On Fri, Apr 27, 2012 at 5:07 AM, 于洪军 wrote:
>
> Hi,
>
> I am trying to screen crystals of membrane p
Hi, Alex:
> Which sigma do you mean?
The one for automatic weight, not for Jelly-body refinement.
I did not turn the "Jelly-body refinement" on.
Thanks
Ros
On Thu, Apr 26, 2012 at 4:08 PM, aaleshin wrote:
> Hi Uma,
> Which sigma do you mean? The one for Jelly-body refinement?
> J-B sigma=0.
Dear All:
I use Refmac5 to refine my structure model.
When I set the sigma value to 0.3 (as recommended from tutorial), the
resulted model has many red-bars by coot validation (geometry, rotamer,
especially, Temp Facotr).
I then lower the sigma value to 0.1, the resulted model is much improved b
How about plain old BSA ?
Jürgen
On Apr 26, 2012, at 1:29 PM, Peter Hsu wrote:
Hi all,
Sorry for the very off topic problem. I'm looking for a loading control for my
westerns (doing in vitro assays). Due to separation/antibody specificity
issues, I need super good separation between 70 and 10
Hi all,
Sorry for the very off topic problem. I'm looking for a loading control for my
westerns (doing in vitro assays). Due to separation/antibody specificity
issues, I need super good separation between 70 and 100 kDa, oftentimes running
those markers all the way to the bottom of the gel. If
Dear Crystallographers,
is anyone aware of any attempts to crystallize membrane proteins in reverse
micelles? Seems like a pretty reasonable route for really greasy membrane
proteins, at least at first blush. I have seen some NMR studies in reverso,
but no crystals
Jacob
***
Dear Naveed,
If your final model fits the final 1.7 Å electron maps density extremely well,
without residual positive or negative difference density peaks, it almost
certainly correctly describes your data. In the case Dale sent us, the fit was
very bad. That the initial density was not that gr
This the last reminder about the BCA/CCP4 Summer School to be held at
Diamond (UK) from Tuesday 28th August to Sunday 2nd September 2012.
Applications ***and referee reports*** are due by next Tuesday, 1st May
2012, so please chase your supervisor!
For more information see:
http://www.diamon
Dear Jeremy,
Thank you for the attached cartoon, most warmly welcome by all those in
need of a "displacement activity" in this gruesomely cold and rainy month of
April.
Oh those terrible French! I know them, I am one of them ;-) .
I found the Wikipedia entry on the subject qu
Dear Colleagues,
I have followed this thread with great interest. It reminds me of the Open
Commission Meeting of the Biological Macromolecules Commission in Geneva in
2002 at the IUCr Congress. Ie at which it was concluded that protein
coordinates and diffraction data would not be provided to r
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