How about plain old BSA ? Jürgen
On Apr 26, 2012, at 1:29 PM, Peter Hsu wrote: Hi all, Sorry for the very off topic problem. I'm looking for a loading control for my westerns (doing in vitro assays). Due to separation/antibody specificity issues, I need super good separation between 70 and 100 kDa, oftentimes running those markers all the way to the bottom of the gel. If I'm careful, I can usually keep the 55kDa marker still on the gel, but my loading control is smaller and runs off. Does anyone know of a commercially available protein that's relatively cheap, has an antibody against it, and is either between 55-70kDa, or >100kDa to add into my experiments so I can have a loading control? Thanks and sorry again for the off topic question. All the best, Peter ...................... Jürgen Bosch Johns Hopkins University Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Office: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-2926 http://web.mac.com/bosch_lab/
