Sure, we can talk about this off line.
-- Kevin
On Thu, Mar 8, 2012 at 7:48 PM, Pius Padayatti wrote:
> Kevin,
> thanks.Sine this is of not much interest to many here
> please feel free to go off line in the future.
>
> The method of dialysis is confusing here.
> How can one achieve this by b
Kevin,
thanks.Sine this is of not much interest to many here
please feel free to go off line in the future.
The method of dialysis is confusing here.
How can one achieve this by buffer exchange?
I wonder if an extensive wash of protein on a column would work
instead.
Pius
On Thu, Mar 8, 2012 at
Was this crystal shot frozen? With new crystals it is always a good idea to
take some room temperature shots first to get an idea of how good the
diffraction could possibly be. If you shoot the crystals at room temperature
and the spots look fine, then the obvious answer is to optimize the freez
In my case, this method was developed for large quantity sample
(~100g/batch) purification.
Under cGMP, I could not introduce EDTA or other chemicals like
Triton-X100 into the system. Otherwise, I just solve small problem but
bring into an even large problem to the manufacture line.
I hope you ca
To Pius,
In my case, the protein/biopolymer is Lysine/amine riched.
1. Lys or Arg are mainly positively charged under pH 7.0 ~ 8.0, and
then provide positively charge to LPA. Of course, some metal cations
could also be involved in LPA binding.
2. Tris with the NH2 group could also be protonated
This is in response to a comment to this thread
Kevin,
Could you explain how that worked?
How do you know your method worked?
Did you estimate the lipopolysaccharide before and after the method?
The method already mentioned here to wash using TritonX100 makes sense.
by washing bound protein sample
Dear Florian,
Could it be that you only have spots in the center of your image and that XDS
is trying to process a lot of image area with only background in it? In this
case it will try to build profiles from non-existing reflections and start to
complain. The resulting bad data may cause scal
Dear All,
I am getting a warning message in XDS I have not seen before, when
trying to integrate a low resolution (~ 7 A) dataset.
!!! WARNING !!! REFERENCE PROFILE # 1 IS EMPTY.
THE AVERAGE PROFILE IN THIS BATCH IS USED INSTEAD.
and so forth for the other reference profile
Dear All:
I appreciate for your comments and inputs.
Thank you
Uma
On Thu, Mar 8, 2012 at 12:16 AM, Shekhar Mande wrote:
> Welljust to add, it has been our contention that many of the metal
> ions have been modelled as waters in several structures- due perhaps to the
> lack of sufficiently
I have changed the GUI default to be MAKE HYDROGEN ALL. This should be
in the next release.
In any case, the option is in the folder Refinement Parameters, so you
can check.
m
On Wed, 2012-03-07 at 23:45 +, Garib N Murshudov wrote:
> Hi
>
>
> 1) refmac's default option has been "generate a
Friends and colleagues of Professor Dame Louise Johnson will be very saddened
to learn that she suffered a serious and very incapacitating heart attack with
complications in August 2011 and has been in hospital since then. Louise is
visited daily by members of her family, and, starting very rece
Hi all,
I apologize for being late in expressing my gratitude. Thanks to Prof. Tim
and Prof. Herman, I have fixed the waters and validated the structure.
Cheers,
ARKO
On Wed, Mar 7, 2012 at 8:37 PM, Tim Gruene wrote:
> -BEGIN PGP SIGNED MESSAGE-
> Hash: SHA1
>
> Dear ARKO,
>
> you can
Mark,
I am faced with the same problem. I work under Linux.
Since everything depends from the .CCP4 directory,
this directory can be copied to
a storage location. Example: /oldproj/dotCCP4_2011:
/home/user % cp -r .CCP4 /oldproj/dotCCP4_2011
Once this directory has been effectively backed up
Dear Jerry,
I've just become aware of this spin column to remove endotoxin, marketed by
Generon, UK.
http://generon.co.uk/index.php?route=product/category&path=238
Good luck,
Jon
On Wed, Mar 7, 2012 at 9:50 AM, Jerry McCully
wrote:
>
>
> Dear All;
>
>
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