[ccp4bb] Position available: Research Technician, High Throughput Crystallisation

Dear All, A Research Technician position is available at the High Throughput Crystallisation laboratory of the EMBL Grenoble Outstation. You will find the details in the link below. http://www.embl.de/aboutus/jobs/searchjobs/index.php?newlang=1&newms=sr&searchregion=670

Re: [ccp4bb] Expression of Viral proteins for crystallography

We've seen nice in vivo activity (on purpose) from proteins cloned under T7 promoters but transformed into non-DE3 cells. In fact, friends working with more zesty enzymes who wanted a more "tunable" in vivo assay have had to mutate the ribosome binding sites for proteins under T7 promotors to k

Re: [ccp4bb] Expression of Viral proteins for crystallography

Toxicity of target proteins in pET vectors can manifest itself without DE3. Some people suggest E. coli polymerases causes low level of expression. The observed mutation rate is thousands of fold higher than what the textbooks say. It’s easy to tell toxicity from other causes of heterogeneity. Less

[ccp4bb] British Crystallographic Association

Dear Crystallographer Following my pitch on behalf of the British Crystallographic Association (BCA) at the 2012 CCP4 Study Weekend in Warwick, I wanted to encourage any interested macromolecular crystallographers to become members of the Association. The UK will host the European Crystallographi

[ccp4bb] BioSAXS Essentials III Workshop

MacCHESS is pleased to announce that the BioSAXS Essentials III Workshop will once again be offered at Cornell University. *BioSAXS Essentials III: Getting Started in Biological Small-Angle X-ray Solution Scattering* * *Date:*February 24-26, 2012 *Location:*Cor

Re: [ccp4bb] Expression of Viral proteins for crystallography

Dear Gregory and Darren, Thank you for your answers. They have been very useful. 2012/1/24 Gregory Verdon > > it possible that the protein is toxic (even when slightly expressed from > your possibly leaky pET vector), so that e.coli select for mutations that > kill expression of your recombinant

Re: [ccp4bb] writing scripts-off topic

Hi Yuri, To add something nobody else has mentioned yet. If you are interested in statistics and analysis you may find interesting the R package. There is an excelent module, called Bio3D for the analysis of protein structure and sequence data. Have a look here: http://bio3d.pbworks.com/w/page/78

[ccp4bb] Beamline Scientist position in SAXS@EMBL Hamburg

Dear all, A beamline scientist position is available at the EMBL Hamburg Unit in Small Angle Scattering in the research group of Dr. Dmitri Svergun. I attach the Vacancy Notice below. Deadline for application is 4th March 2012. Applications should be directed to:www.embl.org/jobs regards Margre

Re: [ccp4bb] writing scripts-off topic

Hi Yuri, If you don't like Python, like myself (and I'm not alone, it would seem), you could try Ruby (http://www.ruby-lang.org/en/). Some examples of PDB file manipulation are below (taken from [1]). The language is a great improvement in Perl and Python in my opinion, but the downside

[ccp4bb] Fwd: [ccp4bb] quasispecies

Begin forwarded message: Date: January 24, 2012 7:16:42 PM EST To: Bart Hazes mailto:bha...@ualberta.ca>> Subject: Re: [ccp4bb] quasispecies This remark brings to mind a paper published recently by Ariel Fernandez and Mike Lynch: Nature 474:502, 2011: Non-adaptive Origins of Interactome Comp

[ccp4bb] Postdoctoral position in Oxford Biochemistry

Dear all, A postdoctoral position is available in Oxford Biochemistry for combined crystallographic and NMR studies of centriolar proteins. The advert is below and applications can be made online until Feb. 20th. Regards, John Vakonakis -- Dr. Ioannis (John) Vakonakis Wellcome Trust Research

[ccp4bb] Introducing an ELN

Tassos reports: > 1. None of the twenty test-users was satisfied with any of the two > solutions - and each was annoyed for a different reason. This suggests that the choice of ELN is not the most difficult part of the adoption process. Maybe the test users at the NKI were annoyed by the idea of

Re: [ccp4bb] quasispecies

On Jan 24, 2012, at 10:31 PM, Bart Hazes wrote: > For some RNA viruses the rate of mutation is so high that they basically > sample a flat region of the fitness-landscape. If you could take two > individual viruses out of this sample to establish two independent infections > than over time eac

Re: [ccp4bb] Problem with getting Rfree and Rf down

Le 24/01/12 21:18, Dale Tronrud a écrit : > On 01/24/12 11:52, Miguel Ortiz Lombardia wrote: >> El 24/01/12 18:56, Greg Costakes escribió: >>> Whoops, I misspoke... I meant Rsym and Rmerge increase with higher >>> redundancies. >>> >> >> But then suppose that one merges data from a crystal that is

Re: [ccp4bb] New restraints, same name

That is the plan and hopefully with help of people from pdb coot, buster, phenix, refmac, ccp4 programs will all use mmcif with ligand descriptions inside. Good will is there, a little bit work is needed. Regards Garib On 25 Jan 2012, at 08:15, Ingo P. Korndoerfer wrote: > ... and why don't w

Re: [ccp4bb] New restraints, same name

... and why don't we move on from pdb format to something a bit more modern ? i think it would only require a handful of programmers to agree on this and the rest would (have to) follow. i.e. if coot and refmac could both read and write something more modern we'd have a huge part of ccp4 world co