Dear all,
Please see the job advert for an EM programmer below.
All responses/inquires to: sche...@mrc-lmb.cam.ac.uk
---
At the MRC Laboratory of Molecular Biology in Cambridge we are looking
to recruit a permanent Programmer to be respons
Bill
So eloquently put, much of what you say resonates with me. Very sad day.
Cheers
Ashley
Sent from my iPhone
On 06/10/2011, at 3:13 PM, "William G. Scott" wrote:
> On Oct 5, 2011, at 5:52 PM, Bosch, Juergen wrote:
>
>> http://www.apple.com/stevejobs/
>>
>> May innovation continue to lead t
On Oct 5, 2011, at 5:52 PM, Bosch, Juergen wrote:
> http://www.apple.com/stevejobs/
>
> May innovation continue to lead the future.
>
> Jürgen
I've been quite saddened about this all evening. Even though we knew it was
coming, it is still incredibly sad, especially at his age (only 56).
Alth
http://www.apple.com/stevejobs/
May innovation continue to lead the future.
Jürgen
..
Jürgen Bosch
Johns Hopkins University
Bloomberg School of Public Health
Department of Biochemistry & Molecular Biology
Johns Hopkins Malaria Research Institute
615 North Wolfe Street, W8708
Hello Jan,
I've used both with soluble and membrane proteins happily. I haven't
seen significant differences with protein behavior or performance in one or
the other, but of course, your favorite protein may differ. I've found that
buffer condition is a more important variable than membrane t
If I'm not mistaken it is caused by /tmp/'username' not existing or
being writable...
Jon
--
Jonathan P. Schuermann, Ph. D.
Beamline Scientist
NE-CAT, Building 436E
Advanced Photon Source (APS)
Argonne National Laboratory
9700 South Cass Avenue
Argonne, IL 60439
email: schue...@anl.gov
Tel: (6
Hi All,
The protein engineering department at Zymeworks Inc. (www.zymeworks.com),
is actively searching for talented structural biologists who are interested
in doing in silco protein engineering.
If interested please visit [http://zymeworks.com/careers/postings.html]
Cheers,
Paula Lario
On 10/05/2011 03:42 PM, Peter Canning wrote:
When I run Parrot to do density improvement and NCS averaging, Parrot
works beautifully (final FOM is 0.87) but NCS averaging causes the
average NCS correlation coefficient to drop (from 0.94 to 0.64) and the
average mask volume to increase from 0.31 t
Dear Herman
In some cases density modification starting with MR phases can indeed prove
very powerful.
We recently used Parrot to bootstrap a difficult case at 4.3 angs and were able
to obtain very good maps for entire missing domains.
See Supplementary Fig 1 in:Verstraete et al 2011 BLOOD 118:
Dear CCP4'ers,
I do have a similar case with 8 molecules in the asymmetric unit,
related by improper symmetry and the structure is solved by molecular
replacement. I do have 2 questions:
1) Does it make sense to average to improve phases? The molecular
replacement phases perfectly obey the non-
Dear CCP4'ers,
I am working on refining the structure of a protein complex consisting of two
different chains. Data were collected to 2.3A in space group C2 and phased by
molecular replacement without any problem, but the ASU contains 6 complexes (so
12 chains in total). In the ASU, the 6 compl
On Wed, 2011-10-05 at 10:36 +0800, Jobichen Chacko wrote:
> I looked into our previous posts and tried to locate the ccp4.LOCK
> file, but I cannot find one in my system.
Curiously, neither can I with the ccp4i currently running. Maybe this
info is outdated, and you should try deleting "database
Adam, sorry I don't use overlapmap, for the reasons you mention (and many
others!). In fact I decided it was in such a mess that it was irrecoverable
& so I wrote my own program EDSTATS to do all all these electron density
stats (and more). I talked about it at the last CSW (
http://www.cse.scite
On Tue, 2011-10-04 at 15:25 -0700, Jun Liao wrote:
> I want to calculate the surface curvature for my proteins in a
> quantitative way and show the results in a graphics software such as
> Pymol
Surface Racer
http://apps.phar.umich.edu/tsodikovlab/index_files/Page756.htm
will output curvature p
The vivaspins are the best thing in
ultrafiltration since sliced bread. I learned about them while I
was at the NIH several years ago and haven't looked back. Fast and
low-binding. PES membranes are superior for minimizing protein
binding, and are also less subjec
Hi Jan,
In our lab we use both the Amicon Ultras and the Vivaspins (soluble and
membrane proteins).
What I can tell you is that me and a few other colleagues had the
experience (and still do) of specific soluble proteins precipitating on
the Cellulose membrane
of the Amicon. By switching to
Hi Jan
I have used Vivaspins for membrane proteins and they generally work fine. The
exact pore size might be different though between Vivaspin and Amicon with the
same specifications, say e.g. 100,000 MWCO. So you might find that your protein
is retained in one of them, while it is in the flow
On Wed, Oct 5, 2011 at 11:03 AM, Adam Ralph wrote:
> Hi Brigitte,
>You are correct, the columns labeled Fobs and Fcal refer to density. The
> columns should be: averaged density for Obs and Cal for the main chain, then
> averaged density Obs and Cal for the side chains. I have included a vers
Dear bbers,
Incase the (extremely attractive) lectureship advertised by Rick is a little
rich for your blood, I'd like to draw your attention to postdoctoral positions
available in the Endicott and Noble groups, currently being established in the
Northern Institute for Cancer Research in the Me
Hi Brigitte, You are correct, the columns labeled Fobs and Fcal refer to density. The columns should be: averaged density for Obs and Cal for the main chain, then averaged density Obs and Cal for the side chains. I have included a version of overlapmap that calculates the R-factor correctly and h
Hi Jan,
I've used both, and I've used them on membrane binding proteins,
extracellular matrix proteins and intracellular proteins.
I know of some cases where protein loss is very severe, but that varies on a
case-by-case basis. A general observation is that proteins with which losses
are bad, are
dear BBers
an opportunity has arisen in newcastle for a lecturer in macromolecular
crystallography. the postholder would either share lab space with my group, or
occupy space in the lab next door that we have spread into recently. to see
what things we have been up to, have a look here: http:sb
Dear all,
sorry, for the slightly off-topic theme, but I wonder if anyone has
compared the above mentioned ultracentrifugation devices, thoroughly.
Currently, we are using the Amicon Ultra, but as the Vivaspins are
considerably cheaper we are considering to change.
I used both with extracellula
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