发自我的 iPad
在 Oct 2, 2011，4:22 PM，Roger Rowlett 写道：
> You can't change the pKa of CO2, which is 6.3. Any attempt to grow bicarb
> complexes below pH 7.5 will be problematic due to CO2 bubble formation, which
> may crack the crystal. What we do in these situations is to soak crystals in
> a cr
You might want to consider misusing the Xe-chamber which a couple of labs and
synchrotrons bought in the late 90's to derivative crystals with Xenon. Instead
of flushing the chamber with Xenon you could flush it with CO2 then plunge
freeze your crystals within the chamber.
careful advertisement
Jacob Keller wrote:
Dear Crystallographers,
I would like to soak my crystals in bicarbonate (a possible
substrate), but the crystals have grown--and only grow--in pH 5.2-6.0,
so the bicarb/CO2 will just keep evolving out of the solution and
reliquishing its hydroxyls until the pH is elevated suf
You can't change the pKa of CO2, which is 6.3. Any attempt to grow bicarb
complexes below pH 7.5 will be problematic due to CO2 bubble formation,
which may crack the crystal. What we do in these situations is to soak
crystals in a cryo solution at a higher pH for as long as practical, then
transfer
===
SYNCHROTRON BEAM TIME FOR MACROMOLECULAR CRYSTALLOGRAPHY AT SLS
===
Proposal application deadline: Saturday, October 15, 2011
Periods:
January 1, 2012 - Ap
Dear Crystallographers,
I would like to soak my crystals in bicarbonate (a possible
substrate), but the crystals have grown--and only grow--in pH 5.2-6.0,
so the bicarb/CO2 will just keep evolving out of the solution and
reliquishing its hydroxyls until the pH is elevated sufficiently out
of range
>> There exists a less toxic chemical than EtBr to stain DNA: SYBR safe
>> DNA stain (a fluorescence dye sold by a certain vendor).
>
> SYBR Safe is about 10X less sensitive though.
Can you do the toothbrush test with SYBR Safe?
I wouldn't do that. As it is considerably more hydrophobic, I'd ex
i wouldn't recoommend that. here is the info from somebody forwarded
from our genetics department with regards to safety of that while
back
"Sybr is just as toxic/poisonous/harmful as ethidium bromide, only
far more expensive. There have been very few tests concerning it's
use up unti
>> There exists a less toxic chemical than EtBr to stain DNA: SYBR safe
>> DNA stain (a fluorescence dye sold by a certain vendor).
>
> SYBR Safe is about 10X less sensitive though.
Can you do the toothbrush test with SYBR Safe?
JPK
***
Jacob Pearson Kell
There exists a less toxic chemical than EtBr to stain DNA: SYBR safe
DNA stain (a fluorescence dye sold by a certain vendor).
SYBR Safe is about 10X less sensitive though.
I suspect that not many chemicals in the lab are less toxic/mutagenic than
EthBr. The classic Ames test shows that 5 ug of
There exists a less toxic chemical than EtBr to stain DNA: SYBR safe
DNA stain (a fluorescence dye sold by a certain vendor). Another
benefit is to be able to use blue light, reducing UV/VIS light
exposure when handling gels.
Florian
On Oct 2, 2011, at 11:49 AM, Edward A. Berry wrote:
Ja
Jacob Keller wrote:
I actually looked at an EtBr MSDS a while ago, and was shocked at how
benign it was. I also heard from someone that they used to feed it to
Argentinian cows routinely a few years back...
Wikipedia says it was used as a trypanosomacidal - It's being
discontinued not because
We chewed pills with EtBr as kids in school to see if we brushed our teeth well
- red colour on the edges, bad boy
Poul
On 01/10/2011, at 19.12, Jacob Keller wrote:
> I actually looked at an EtBr MSDS a while ago, and was shocked at how
> benign it was. I also heard from someone that they us
1-So for my case, I should try and generate Rfree flags, in the
higher apparent C 2 2 21 and not the real P 1 21 1, correct?
2-I noticed
that if I used the output PHIC FOM data, the maps looked awesome! Too
good almost...
Thanks a lot
On Sun, 2 Oct 2011 12:07:22 +0100, Garib
N Murshudov wro
Post-doctoral position: structural studies of nucleotides carriers
Location: IBS, Grenoble, France
Starting date of the contract: latest 1^st of December 2011
A post-doctoral position is available to join the group of Pr. E.
Pebay-Peyroula at IBS (Institut de Biologie Structurale, Grenoble,
Fr
Dear Yuri
1) For twin refinement refmac internally changes free reflections so that twin
related reflections belong to the same class (twin or working). However it
would be good to generate free reflections in higher space group that would
include twin and space group symmetries.
2) You should
16 matches
Mail list logo
