Typically, what you image is Trp fluorescence by exciting at around 280 nm
and observing at around 350 nm. Standard silicon based detectors do fine at
the detection wavelength, although, as you can imagine, increased
sensitivity in the UV means increase in the price of the detector. If your
excitat
Dear all,
I have two questions:
First, I was trying to crystallize a complex of two proteins. Both proteins has
been crystallized before. The two proteins bind to each other based on Biacore
study, but they didn't form a single peak on gel filtration. When I mixed them
at 1:1 ratio, the cry
A "real" UV microscope requires quartz optics, right?
Probably conventional microscopes use glass.
And you can't see 280 nm (and its not good for your eyes)
so you need some kind of phosphor screen to view the image?
Bosch, Juergen wrote:
I'm replying here to myself :-)
So in an off-board discu
I'm replying here to myself :-)
So in an off-board discussion it turns out that the "microscope" in question
was a special emitted light and not a UV microscope. So real UV microscopes
might be better for the purpose of detecting real crystals.
Sorry for the confusion - had too much sun today :
Dear Jacob,
agree, it's a mess. From what I read, the glutataldehyde concentration should
be low (<0.01%) and the x-linked complex that you get should not occur in high
salt conditions (reasoning that 1.2M KCl would break the average complex
apart). Have seen papers where more selective zero le
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Applications are invited for two Ph.D. positions to study the
structure and function of macromolecular complexes involved in
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To understand mechanisms underlying asymmetric
>> Maybe one should do a gradient of
>> gluteraldehyde concentrations, then plot the deviation of the observed
>> cross-linked oligomerization from a theoretical null hypothesis?
>
> Right - just do it side-by-side with a protein known to be monomeric of
> roughly the same size/lysine content... A
Quoting Ed Pozharski :
On Thu, 2011-09-15 at 20:50 +0100, Andrew Purkiss-Trew wrote:
Molecular Dimension do such an adaptor which fits to existing
microscopes.
Do you by any chance know the price? I can seemingly "order" it through
the website for the hefty price of $0.00, which is too good
I once tested such a commercial system in Seattle about 4 years ago. It did not
impress me. In particular the discrimination between salt and protein did not
work for about 10 different proteins from which we already had collected data.
sure those were small between 10 and 100 micrometer. Excuse
On Thu, 2011-09-15 at 15:10 -0500, Jacob Keller wrote:
> Maybe one should do a gradient of
> gluteraldehyde concentrations, then plot the deviation of the observed
> cross-linked oligomerization from a theoretical null hypothesis?
Right - just do it side-by-side with a protein known to be monomer
Dear Crystallographers and Biochemists,
cross-linking, say with gluteraldehyde, is an oft-used method of
demonstrating a protein's oligomeric state in solution. I have a
difficulty with this, however: theoretically (and in practice!), one
can tune the amount of cross-linker to get what ever result
A while ago I was trying to be cheap, so we played around with it quite
a bit in the lab. After rediscovering some of the basics of
signal-to-noise and microscope transmission efficiency and that sort of
rot, I realised that the commercial systems may not be all that
ridiculously overpriced af
On Thu, 2011-09-15 at 20:50 +0100, Andrew Purkiss-Trew wrote:
> Molecular Dimension do such an adaptor which fits to existing
> microscopes.
Do you by any chance know the price? I can seemingly "order" it through
the website for the hefty price of $0.00, which is too good to be true.
--
"Hurry
Quoting "Harman, Christine" :
Hi All,
I was curious if any of you have tried or even know if it is
possible to adapt a stereoscope (in my case an Olympus SZX10 model)
so as to view protein crystals with UV illumination. Basically, I
want a cheap manual version of what a Rock UV Imager does
I'm not going to respond to the larger group, but I know one can buy LEDs
that emit strongly at 280 nm, which would give tryptophan fluorescence.
They're about $200, and one could build or buy a control circuit for not
much more. I think this is about what the commercial tools do. You'd
want
Hi All,
I was curious if any of you have tried or even know if it is possible to adapt
a stereoscope (in my case an Olympus SZX10 model) so as to view protein
crystals with UV illumination. Basically, I want a cheap manual version of what
a Rock UV Imager does. I know this is probably a crazy d
CCP4 Study Weekend - 4-6 January 2012
We cordially invite you to participate in this year's Study Weekend at the
Warwick Conferences, University of Warwick. Once again, we have put together an
exciting scientific programme for Thursday and Friday, either side of the
traditional conference dinne
Hi Pascal,
The map data is a three dimensional array with dimensions [NC, NR, NS]. On
its own, this gives you no information about the grid pitch in the three
(crystallographic, not Cartesian) directions, which is determined in
fractional coordinates by the number of intervals. That is, along the
typo: MAPC, MAPR and MAPS are elements 17-19 of the header, but you can see
that anyway from the specification.
Cheers
-- David
On 15 September 2011 09:51, David Waterman wrote:
> Hi Pascal,
>
> The map data is a three dimensional array with dimensions [NC, NR, NS]. On
> its own, this gives yo
Hello Alex,
I know nothing about Procheck but the following may be of interest to you.
There is a similar error message produced during ARP/wARP model building as
described in the message below:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?A2=CCP4BB;30c74e5a.1109
This error was found to be cau
Hi,
I am looking at the specifications of the ccp4 map file format and I am
confused with the number of columns and the number of intervals.
I assume that the number of columns is the grid size but what is the
number of intervals (elements 8-9 in the header)?
Regards,
Pascal
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