According to your link it's not worth it right ? I mean lion taming ?
Jürgen
On Jul 22, 2011, at 6:59 AM, Gerard DVD Kleywegt wrote:
Hi all,
Just a heads-up - if you upgrade to OSX Lion, your trusty old USF executables
will no longer work.
Help is on its way - in the near future the indefatigab
Dear all,
we're in urgent need to replace an old Precision Scientific Low Temperature 815
Incubator. We could quickly get a VWR Symphony Low-Temperature BOD Incubator
but before purchasing I was wondering if anyone has any experience with this
incubator for protein crystallization.
I'd apprecia
Dear All,
We're very pleased to announce the release of the latest version of the CCP4
software suite. Version 6.2.0 (Giggleswick) is now available from the CCP4
download website:
http://www.ccp4.ac.uk/download.php
The release is available for Linux, Mac OSX and Windows platforms. Updates
inc
I recall using CME some time ago (within last couple of years) without
problems. Also, an alternative approach is to incorporate BME as a
separate residue instead - the disulfide bond will be correctly
identified by refmac. I would think ideologically this is more
appropriate - using a different
As Lion seems to be, above all, an wonky iPad emulator, you're probably
better off keeping the Snow Leopard alive until Apple obsolesces it in a
few months.
Andreas
On 22/07/2011 11:59, Gerard DVD Kleywegt wrote:
Hi all,
Just a heads-up - if you upgrade to OSX Lion, your trusty old USF
ex
Can I use the Linux version under Xterm (from Macports)? I noticed that the
Fink repository will not run on the Lion system, but it looks like Macports
is OK, because they have a new branch for Lion. And they also have a dmg
install file for Lion.
On Fri, Jul 22, 2011 at 12:59 PM, Gerard DVD Kleyw
Hi all,
Just a heads-up - if you upgrade to OSX Lion, your trusty old USF executables
will no longer work.
Help is on its way - in the near future the indefatigable and intrepid
Lion-taming heroes of the USF will release a distribution kit that includes
source code. However, for now you may
Subbu,
Don't forget you crystallization screen can also be used to find a
condition that your protein may be happier in ! As you already have setup a
screen of your protein, have a look at your drops again and see if there
are any conditions that are clear. If so, is there a consistent theme
The self rotation isnt the correct input to the translation function -
just run the Auto-Molrep option..
Eleanor
On Thu, 21 Jul 2011 20:57:02 +0800, Hubing Lou
wrote:
> I also processed with Imosflm and ran Pointless, it was P21. Also it was
> indicated by the "Intensity systematic absences" in H
A solution could be to control the solubility of your protein in different
pH and salts (you can also add some additives) by using the Thermal Shift
Assay. You may find a better buffer in which to concentrate your protein.
If you think that the reason of the low diffraction is the quality of your
Dear AfshanMake sure that group name for CME is peptide (or L-peptide). In the new version CME is peptide. I am not sure it was the case in older versions. I attach CME as a peptide. You can add this into your dictionary.Then CME can become part of a peptde. It would also be good to remove all the
Dear Afshan,
I have been struggling with the same irrating "feature" (I would call it
a bug), which was for me one of the reasons to abandon refmac. For some
reason and against PDB standards, refmacs wants linked amino acids to be
consecutively numbered and otherwise decides that they are not lin
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