Dear All,
Is anyone using ICP 700 (416 Litres) Memmert incubators for protein
crystal growth? What are their experiences?
Are they vibration free as much as 3201 (390 Litres) Rubarth Rumed
vibration free incubators ?
I know memmert has low volume which are peltier cooled incubaters,
no
Hi there,
I would like to know how to convert a .osc file to
.img file which can be then read by mosflm or ccp4 programs so that it
can be indexed and integrated.
Thanks!
Madhu
Thanks all! Actually I have tried different systems and did find that the
"molecular envelop" map shows up at a resolution of 40A (50S ribsome).
Hailiang
> Hi Hailiang,
>
> may be your expectations did not match the reality? I did exactly this for
> teaching purposes a few weeks ago and it worked
Are you doing MR at low resolution or just cuting data to compare with an EM
map?
Sent from my iPhone
On Jun 1, 2011, at 7:35 PM, Hailiang Zhang wrote:
> Hi there,
>
> I have a preliminary question. For very low resolution data, say 10A or
> even lower, is the density map supposed to be more
Hi Nat,
playing with fake data you know the answer. Real data are good too, although
you have to keep in mind how its completeness will affect what you look at.
I can't see how B-factors are relevant if you take a model from PDB: in this
case the B-factors are what comes from PDB - real ones.
Pav
Hi Hailiang,
may be your expectations did not match the reality? I did exactly this for
teaching purposes a few weeks ago and it worked for me quite well.
Try different models with different molecule/solvent ratio, try adding
solvent:
phenix.fmodel model.pdb high_res=10 k_sol=0.35 b_sol=50
Yes,
On Wed, Jun 1, 2011 at 4:45 PM, Pavel Afonine wrote:
> to get feeling about how maps may look like at different resolutions do the
> following learning exercise: download a structure from PDB and compute Fcalc
> maps at different resolutions:
>
> phenix.fmodel model.pdb high_res=1
> phenix.fmodel
On Wednesday, June 01, 2011 04:45:36 pm Pavel Afonine wrote:
> Hi Hailiang,
>
> to get feeling about how maps may look like at different resolutions do the
> following learning exercise: download a structure from PDB and compute Fcalc
> maps at different resolutions:
>
> phenix.fmodel model.pdb h
Hi Pavel:
I tried phenix.fmodel at different resolutions up to 20A, and never got
any big envelop covering the whole molecule.
...anyway, thanks...
Hailiang
> Hi Hailiang,
>
> to get feeling about how maps may look like at different resolutions do
> the
> following learning exercise: download
Hi Hailiang,
to get feeling about how maps may look like at different resolutions do the
following learning exercise: download a structure from PDB and compute Fcalc
maps at different resolutions:
phenix.fmodel model.pdb high_res=1
phenix.fmodel model.pdb high_res=2
phenix.fmodel model.pdb high_r
Hi there,
I have a preliminary question. For very low resolution data, say 10A or
even lower, is the density map supposed to be more like a big envelop
covering the whole molecule, or more like a collection of isolated small
envelops covering small motifs (eg helix as cylinder envelop)? I got the
Many of you will have seen the request for expressions of Interest for
the phase III proposal for "A versatile microfocus beamline with an
in-situ side station for macromolecular crystallography at DIAMOND".
As one of the co-proposers I would like to urge you to support this
proposal and write a l
Hi Li Chen,
try
#!/bin/sh -f
sc xyzin 1XOU.pdb << eof-sc
Molecule 1
CHAIN A
MOLECULE 2
CHAIN B
end
eof-sc
in a small script lets say "sc.com"
then run %sc.com |tee sc.log
and you'll get something like this :
Summary of results:
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