Hi all,
I have been asked to pass on the following details for a position at the
University of Sydney. Please address all correspondence to the University of
Sydney as detailed below.
Cheers,
Aaron
LECTURER/SENIOR LECTURER IN STRUCTURAL BIOLOGY
SCHOOL OF MOLECULAR BIOSCIENCE
REFERENCE NO.
Stefan
A second go!
Can you compare the spacings of some of the rings, including the ones showing
partial alignment, with the ones in this picture.
http://www.pnas.org/content/102/2/315/F2.large.jpg
PNAS January 11, 2005 vol. 102 no. 2 315-320
Molecular basis for amyloid fibril formation an
I should point out that the reference I gave is quite misleading as the samples
studied contained lecithin
Apologies
Colin
> -Original Message-
> From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
> Colin Nave
> Sent: 06 April 2011 18:33
> To: CCP4BB@JISCMAIL.AC.UK
>
Hi all,
The following question was posed on the CCP4 bulletin board earlier this week:
I have a question related to protein structure, but not crystallography
per se. Has anyone see a disulfide forming between the two cys of "CXC"
in the middle of a loop, and create a sharp turn, where X is not
First few reflections seem characteristic of lamellar (rather than 3D
crystals). Might be something other than PEG but see
Magda El Nokaly, Stig E. Friberg, David W. Larsen, Lyotropic liquid crystals
from lecithin, water, and polyethylene glycol, Journal of Colloid and Interface
Science, Volum
It seems hard to imagine what there is anything in his solutions other
than protein that would make spherulites, no? PEG 8000 at 12% seems
pretty benign, unless the trays or screens have been sitting around
for quite a while...
JPK
On Wed, Apr 6, 2011 at 12:04 PM, Colin Nave wrote:
>
>
> Stefan
Stefan
Could the low angle reflections be from lamellar liquid crystals of the PEG (or
a mixed PEG/water phase). Seems like you have 1st,2nd and 4th order reflections
with presumably the 2nd order about 20A.
I think 40A lamellar spacing is characteristic of some PEG liquid crystals.
Colin
From
Bernhard,
> Well, "it" *IS* broke.
As they say "it works for me", so either you're using a different set
of programs from me, or you're using the same programs but in a
different way. Perhaps you could be more specific as to which
program(s) appear to be broken? If possible please post the
logf
Dear users,
Is there a possibility that both non-merohedral twinning
and partial hemihedral twinning occur in the same crystal?
In one of the data in R3, sfcheck indicated twinning and thus
upon using twinning option during refinement, refmac considered
twinning operator of (k h -l). But the
Hi Stefan,
IIRC, a ring at 20Å means that one of the cell edges is at least 20Å
long - which might be considered unusually long for a salt crystal -
but it's also on the short side for a protein cell edge - how big is
your protein?
If there are rings at lower resolution, then maybe these longer e
Hi Dave,
thanks for your reply. Is there any chance of telling whether it is salt or
protein from the diffraction image?
Stefan
On Apr 6, 2011, at 12:16 PM, David Briggs wrote:
> Hi Stefan,
>
> It looks like a powder diffraction-type image.
>
> http://en.wikipedia.org/wiki/Powder_diffracti
Hi Stefan,
It looks like a powder diffraction-type image.
http://en.wikipedia.org/wiki/Powder_diffraction
You can imagine that your spherulites are made up of many small
crystals in random orientations, a bit like a powder - this means that
rather than getting discrete diffraction spots as you w
Hey guys,
When I collect data from these spherulites/crystals (grown in 0.1 M sodium
acetate, 0.1 M MOPS pH 7.5, 12 % (w/v) PEG-8000, protein buffer: 100 mM NaCl,
50 mM HEPES pH 7.5):
http://img695.imageshack.us/i/cryst.png/
I get this diffraction pattern: (it's not cryo protected, so there's
Dear all,
of course this may not always be possible, but let me suggest that in
cases such as this, the original poster should consider to make her
dataset (or at least 10 degrees at phi=0, and another 10 degrees at
phi=90) available (by FTP or from a webserver, or even using a publicly
acces
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