Stefan A second go!
Can you compare the spacings of some of the rings, including the ones showing partial alignment, with the ones in this picture. http://www.pnas.org/content/102/2/315/F2.large.jpg PNAS January 11, 2005 vol. 102 no. 2 315-320 Molecular basis for amyloid fibril formation and stability O. Sumner Makin , Edward Atkins , Pawel Sikorski , Jan Johansson , and Louise C. Serpell For a powder diffraction pattern from 3D crystals of a folded protein see for example http://www.aps.anl.gov/Science/Future/Workshops/Biological_Crystallography/Summaries/VonDreele.htm Colin From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Stefan Münnich Sent: 06 April 2011 09:33 To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] diffraction of spherulites Hey guys, When I collect data from these spherulites/crystals (grown in 0.1 M sodium acetate, 0.1 M MOPS pH 7.5, 12 % (w/v) PEG-8000, protein buffer: 100 mM NaCl, 50 mM HEPES pH 7.5): http://img695.imageshack.us/i/cryst.png/ I get this diffraction pattern: (it's not cryo protected, so there's some ice-rings also) http://img683.imageshack.us/i/diffv.jpg/ It can't be only ice-rings because those are usually starting at something like 3.8 A, whereas I already got one ring directly around the beam center and also one at about 20 A. Has anybody seen anything like that and tell me what it is? Stefan
