[ccp4bb] buffers for crystallization

2011-01-26 Thread chandan kishore
Hi Everyone, I have to crystallize one protein which contains a zinc fingure motifs, I am facing a problem in crystallization. I already used 388 buffers . Can anyone suggest me some books or papers freely available online on Buffers required for crystallization. Thanks

[ccp4bb] Phenix version 1.7 released

2011-01-26 Thread Paul Adams
The Phenix developers are pleased to announce that version 1.7 of Phenix is now available. Binary installers for Linux, and Mac OSX platforms are available at the download site: http://phenix-online.org/download/ Some of the new features in this version are: Graphical Interface ---

[ccp4bb] Post-doc position on Validation of Low-resolution Models

2011-01-26 Thread Gerard DVD Kleywegt
Hi all, There is an EMBL Inter-disciplinary Post-doc (EIPOD) position available to work on "Validation of low resolution structural models". For details, see: http://www.embl.de/training/postdocs/eipod/app2011-1/svergun.pdf The post-doc will be based at EMBL Hamburg and supervised jointly b

Re: [ccp4bb] Using Se-labelled peptide to 'deconvolute' pseudo-symmetry

2011-01-26 Thread Herman . Schreuder
Dear Kris Kristal (if this is your real name), As Juergen pointed out, you may suffer from a twinning problem. However I do not think that in your case the twinnig fraction will give any useful information, since the proteins of the dimer will be almost perfectly symmetrical and only the peptid

Re: [ccp4bb] Using Se-labelled peptide to 'deconvolute' pseudo-symmetry

2011-01-26 Thread Bosch, Juergen
What's your twin fraction in each of your anticipated space groups ? That could be the other explanation for your dilemma. Jürgen - Jürgen Bosch Johns Hopkins Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe

[ccp4bb] Using Se-labelled peptide to 'deconvolute' pseudo-symmetry

2011-01-26 Thread Kris
Hi everyone, I have a tricky structure that has pseudo-symmetry. Before we publish the structure, we would really appreciate some valuable feedback from experts like yourself. I have recently solved a crystal structure (2.2 Angstrom) made up of a peptide bound to a dimer. If I solve the struc

[ccp4bb] post a message

2011-01-26 Thread Katrin Rittinger
Could you please post the following message? Thanks A postdoctoral fellowship is available in the group of Katrin Rittinger at the National Institite for Medical Reseach (MRC-NIMR) in London to stud

Re: [ccp4bb] mono S vs. mini S vs. S-HyperD

2011-01-26 Thread Jonas Boehringer
OK, thanks for the answers so far. I think my question hasn't been precise enough. Of course I have already optimised the purification using the Mono S column. However, resolution is not perfect and in the near future I will have to generate a lot of material and would like to use a larger colum

[ccp4bb] Postdoctoral Position at EMBL, Grenoble

2011-01-26 Thread Jose A. Marquez
Dear All, A postdoctoral position is available at the EMBL Grenoble Outstation. You will find the details in the link below http://ig14.i-grasp.com//fe/tpl_embl01.asp?newms=jj&id=41107&aid=15470 Best regards Josan _ Jose A. Marquez Ph.D.