I've used phenix.xtriage and it showed that in all three cases i.e. (P31,P61 &
P6122 space groups) "translational pseudosymmetry is very likely present).
Again, when I used molrep in ccp4,it showed "translational pseudosymmetry is
detected".
Hi all, a symposium announcement:
The 2nd Oxford Symposium on Epigenetic Mechanisms in Health and Disease will be
held 9-10th December 2010 in the Said Business School, Oxford.
The symposium aims to bring together clinical and preclinical scientists
engaged at the forefront of control of infla
posting test
BR
The School of Biological Sciences at the University of Auckland, New Zealand invites applications for a Chair in Structural Biology. The ideal candidate will combine X-ray crystallography with a broad range of biophysical, biochemical and cell technologies to solve relevant biolo
1)My protein has 14 cysteine residues.
>
That can be a big issue in itself :)
> 2)It is not a metal binding protein.
>
Are you sure, with this many Cys? Positive?
> 3)I have added the protease inhibitor cocktail + PMSF during sonication and
> cleavage. I saw only one band of protein bind on b
Hi CCP4BB folks,
I am a freelance protein crystallographer looking to offer advice,
problem-solving (protein purification, crystallization, X-ray data collection,
crystallographic computing and model building etc), analysis of structure-based
projects or provide hands-on assistance.
Based
Hi,
to remove 'some of the voodoo' out of it, we recently published a paper
showing a really fast_and_easy (and cheap) technique to check HA binding
on cysteines.
One of the most interesting result was that we could see binding of
mercury and gold on 1 protein, but not platinum. We didn't try to
e
Dear all,
Sorry about non crystallographic query. Are there any binding studies (using
Biacore) on interactions between antibody and anti-antibody (particularly
mouse antibody)?
thank you
Putcha
Sandy,
like mentioned previously, sounds like FeS.
record an optical spectrum. Or even better, check whether there is
somebody on the campus running an EPR machine (equipped for helium
temperature measurements)
Maybe a new FeS protein? FeS is not necessarily required as redox
cofactor. It can
Paul,
We've been running an Oxford Diffraction (now Agilent) Gemini
system for nearly two years now. We have the older PX Ultra source
for protein, and a Ruby (135 mm) detector. As an educational
institution, we actually do a lot of structure solution in-h
ealrier on this forum Artem have posted about PTM
in bacteria.
this answer your question on PTM in bacteria.
QUOTE
"Yes, this does happen.
Spontaneous α-N-6-Phosphogluconoylation of a "His Tag" inEscherichia
coli:The Cause of Extra Mass of 258 or 178 Da in Fusion Proteins
http://www.sciencedire
How about incomplete TEV cleavage ?
Your protein is a dimer/multimer in solution and some of it is cleaved and some
not and they stick together and are separated on the SDS gel ?
Jürgen
-
Jürgen Bosch
Johns Hopkins Bloomberg School of Public Health
Department of Biochemistry & Molecular Biology
Dear all
Thanks for yours valuable suggestion.
Just some addition information to make the query clear.
1)My protein has 14 cysteine residues.
2)It is not a metal binding protein.
3)I have added the protease inhibitor cocktail + PMSF during sonication and
cleavage. I saw only one band of protein
Do you have translational pseudo symmetry - what is the evidence?
It can confuse space group assignment..
Eleanor
Seema Nath wrote:
After running phenix.xtriage the possible point group is P622 and possible spacegroups
are P622,P6122,P6522,P6222,P6422,P6322. Using this information when I run PH
After running phenix.xtriage the possible point group is P622 and possible
spacegroups are P622,P6122,P6522,P6222,P6422,P6322. Using this information when
I run PHASER, P6322 is shown to be the most probable one.After 5 cycles of
phenix.refine R-& R-free - 57 & 60 respectively.
From other crysta
2 Postdoctoral Research Fellows in
Structural Biology of Hsp90 complexes (Ref 041)
Genome Damage and Stability Centre
School of Life Sciences
University of Sussex, Brighton, U.K.
Two Wellcome Trust-funded Postdoctoral positions are available immediately in
the laboratory of Professor Laurence Pe
16 matches
Mail list logo
