There is an immediate opening for a postdoctoral position in the Goldman group
in the Institute of Biotechnology at the University of Helsinki
(http://www.biocenter.helsinki.fi/bi/xray/goldman/Home_.html). My group
focusses on proteins in or near the cell membrane. In particular, we have a
se
Hi All,
Many thanks for replying to my request on "R-Rfree vs resolution". Your
wonderful advices are very helpful.
2010-08-05
joybeiyang
Dear Changyi
I do not know details about your structure, but considering you have the
correct library for your ligand and your resolution is not bad, I would check a
possible double conformation.
Good luck
Cristy
On Wed, 4 Aug 2010 16:15:55 -0400, Changyi Xue wrote
> Dear all,
>
> In my
A postdoctoral position focusing on structural and functional basis of
cation-coupled symport is available immediately in the laboratory of
Dr. Lan Guan in the Department of Cell Physiology and Molecular
Biophysics at Texas Tech University Health Science Center, Lubbock,
TX. The Guan Lab c
Dear Changyi,
Yes it can. You should have a xxx.cif file which Refmac uses to know
about your ligand. Please have a look at it and check that it is
actually representing the ligand you want to refine. You can
then play with standard deviations in the file to put more weight on
idealised geo
Dear all,
In my structure, there is a ligand, which
contains a sugar ring. In the process of refinement, refmac always
tried to distort the sugar ring to fit into the density. Is there any
way to fix or restrain the ring conformation more tightly? I know CNS
has such function, just wanderi
Yes, Bryan is right. This idea is totally 1970s, but with the smaller
concentrators (microcons) and the smaller spec (nanodrop or equivalent.)
Actually, since you have a lot of protein, you could scale it up more to avoid
needing the nanodrop. But, most departments seem to have a nanodrop somew
Dear Xuan,
I am not certain, but I think that Jacob was referring to a
spectrophotometer called a Nanodrop. It is available from ThermoFisher
Scientific and can provide absorbance data on as little as 2uL of
sample. I think that if you have access to a Nanodrop, and use Ultrafree
0.5mL concentra
However, if the spots of the larger cell are very weak, this means that
the pseudotranslated molecules are very similar and one could save
oneself a lot of pain by just ignoring them. In this case one would just
look at the average of the two pseudotranslated molecules.
Best regards,
Herman
-
I'd expect anything from crystals, and this would not surprise me
either. Especially if it's a big crystal.
That said: if the big cell has pseudo-translational symmetry, it will
have lots of weak spots (?). So getting the "small cell" may just be an
artefact of having very weak diffraction
Dear Jürgen,
Thanks for the feedback.
Differential Scanning Fluorimetry is a nice method but my current goal is to
identify the nature of the cofactor if any. I was wondering whether MassSpec
could be of help.
Sincerely,
Xuan
2010/8/4 Jürgen Bosch
> Check the thermal stability with and witho
Dear Jacob,
Nanodrop ultrafiltration sounds really fancy to me. I am afraid that I have
no access to such equipment yet.
Thanks for letting me know about this new technology.
Sincerely,
Xuan Yang
2010/8/4 Jacob Keller
> I like nanodrop ultrafiltration:
>
> concentrate your protein to the highe
I've got a data at 3.7 angstrom resolution,P3 space group but smallest cell
volume as predicted by marIndex is very large in comparison to the 90 residue
small protein & I'm supposed to solve it using auto-MR and CNS, now when I'm
using auto-MR to search CRF & TF peaks with lowest R-factor & app
I like nanodrop ultrafiltration:
concentrate your protein to the highest stable concentration possible
figure out what is the lowest possible robustly-detectable nadph signal on
your nanodrop
combine the two in such a way in the top of a microcon of appropriate MWCO
to acheive the highest possib
Check the thermal stability with and without your ligand. You could do this via
CD or with the help of Sypro Orange in a RT-PCR machine.
Jürgen
..
Jürgen Bosch
Johns Hopkins Bloomberg School of Public Health
Department of Biochemistry & Molecular Biology
Johns Hopkins Malaria
A Wellcome Trust funded post-doctoral position is available from September in
the Astbury Centre for Structural Molecular Biology at the University of Leeds
in the laboratories of Dr John Barr and Dr T. Edwards. The successful applicant
will study structural and functional aspects of bunyavirus
Dear All,
3D structure modeling server I-TASSER predicts a binding site for NADPH and
I want to test this prediction. What would be the nice quick way to tell
whether this protein bind NADPH or not, when I have a lot of recombinant
protein?
Sincerely,
Xuan Yang
17 matches
Mail list logo
