In the end it comes to the question what added value above the current
approaches you could offer.
If you increase the success rate of crystallizability and good
diffraction data from a given protein by a factor of two or more it
becomes interesting - I am afraid this is not the case for microg
gro
Have a look at this:
http://www.nature.com/nature/journal/v404/n6774/full/404114a0.html
Clemens
Zitat von Jack Reynolds :
TITLE: "Extracting trends from two decades of microgravity
macromolecular crystallization history" (2005) (Judge, Snell and van
der Woerd).
"Significant enhancement
TITLE: "Extracting trends from two decades of microgravity macromolecular
crystallization history" (2005) (Judge, Snell and van der Woerd).
"Significant enhancements in structural knowledge have resulted from X-ray
diffraction of the crystals grown . . . in the reduced acceleration
environnmen
Dear all,
I am working on protein-DNA complex of which I finally got crystals,
which diffract to at about 2 A. Unfortunately I wasn't able (so far)
to solve the structure by MR, since the sequence identity to known
homologues is very low (below 25%).
By now I have successfully produced SeMe
--- On Sun, 5/9/10, Klaus Fütterer wrote:
> Dear Jack,
>
> I believe your venture would enter a mature market, and, if
> you were to offer growing growing crystals in microgravity,
> a market characterised by very high costs and (presumably)
> very low margins.
I wouldn't offer crystal growth,
Dear Colleagues,
The main problem with a lossy compression that suppresses weak
spots is that those spots may be a tip-off to a misidentified
symmetry, so you may wish to keep some faithful copy of the
original diffraction image until you are very certain of having
the symmetry right.
That b
Frank von Delft wrote:
Just looked at the algorithm, how it stores the average "non-spot"
through all the images.
What happens with dataset where the "non-spot" (e.g. background)
changes systematically through the dataset, i.e. anisotropic datasets
or thin crystals lying flat in a thin loop?
This is a purely historical comment. In the 1970's we were faced with the
problem that if, as was then the practice, the reflection data were stored
on punched cards with one reflection per card, even small molecule
structures could be rather heavy to carry around. One of the innovations
introd
Just looked at the algorithm, how it stores the average "non-spot"
through all the images.
What happens with dataset where the "non-spot" (e.g. background) changes
systematically through the dataset, i.e. anisotropic datasets or thin
crystals lying flat in a thin loop? How much worse is compr
Dear all,
Thank you to all those who responded to my question about freezing crystals
grown in isopropanol.
To recap:
- my crystals grew in 25% isopropanol, plus some citrate buffer at pH4.5 (no
other ingredients).
- my crystals grew at room temperature, in 1micro-litre sitting drops, in
Innovap
10 matches
Mail list logo
