Thank you. Forget to mention, no diffraction observed no matter with or without
cyro cooling.
寄件人﹕ "tom.p...@csiro.au"
收件人﹕ theif...@yahoo.com.hk
傳送日期﹕ 2010/4/19 (一) 11:29:38 AM
主題： RE: [ccp4bb] Re： [ccp4bb] Mysterious Crystals?
Hello Tc,
It isn’t that u
Yes, I have just done that. They are protein. But if they are protein, why no
diffraction? That's intriguing.
寄件人﹕ Jürgen Bosch
收件人﹕ CCP4BB@JISCMAIL.AC.UK
傳送日期﹕ 2010/4/19 (一) 10:57:40 AM
主題： Re: [ccp4bb] Mysterious Crystals?
Fish and wash some crystals then ru
Fish and wash some crystals then run them on a SDS-gel, then you will know for
sure if it's protein or not.
Jürgen
On Apr 18, 2010, at 10:46 PM, tat cheung cheng wrote:
> Hi all,
>
> I have got some crystals, the purified protein was in Tris buffer with 300mM
> NaCl for crystallization. they
Hi all,
I have got some crystals, the purified protein was in Tris buffer with 300mM
NaCl for crystallization. they grew in light weight PEG, PEG400 or monomethyl
ethyl PEG500, they were needle shaped, could be long (~0.2mm) but very thin all
the time and sometimes grew into sea-urchin like ne
Dear CCP4bb,
I'm running into some difficulties with Pymol, in particular I want to
visualize via APBS the electrostatic surface of the protein, a protein peptide
and an inhibitor molecule. I can render the electrostatic surfaces for each of
them separately but I fail to show individual surface
Dear Frances,
You set a brilliant example. I spent the last three days stuck in UK after the
BCA meeting and hotels are really not cheap if you don't want to be there in
the first place. Staying at a fellow-scientist's home may be much more
interesting.
That said, I managed to get a seat on the
