Hi, everybody,
We recently recognized a problem with our Innovaplate SD-2 (a.k.a. MRC
2-Well) plates from Hampton. Our last batch of Innovaplates have an
inconsistent well height such that our crystallization robot (a
Mosquito) cannot put protein in all the wells. When tested with an older
In
Dear All,
I have created this website MAHORI (http://felix.bioc.cam.ac.uk) to
display ligand interaction with proteins in the PDB. There is a lot of room
for improvement and I really hope to get feedback from the CCP4 community so
that I can make it more useful to chemical biology, medicinal chemi
Dear Sivaraman
I worked on some protein with DNA contamination recently. Adding DNase
and increasing IMAC wash volume at the same time changed 280/260 ratio
and yield reproducible protein crystals, not high resolution yetIn
case your protein can't stand other treatment...
(Sorry Tommi, I hit
PhD position in crystallization and structure solution of natural and
appropriate variants of the sponge protein silicatein and nanobiotechnology
applications
TOPIC: Research in the field of biomineralization and applications in
nanobiotechnology. Biomineralization is the formation of minera
Dear Claudia,
This could be a good starting point:
Protein Expr Purif. 2008 Apr;58(2):210-21. Epub 2007 Nov 22.
The use of systematic N- and C-terminal deletions to promote production and
structural studies of recombinant proteins.
Gräslund S, Sagemark J, Berglund H, Dahlgren LG, Flores A, Hamma
Dear All,
I'd like to espress a protein for crystallisation as a whole, but also to try
separate expression of its two domains.
Although prediciting domain boundaires is not difficult, I know that it is
worthwhile testing several constructs to improve espression in soluble form. In
s
