Hi Amit,
Normally, we optimize annealing conditions with labeled DNA (cy5, FAM) -
adding MgCl2 and KCl usually improves annealing efficiency. You can
detect fractions of single and double stranded DNA on standard PAGE
gels. We then use the same condition to anneal the crystallization
reagents.
Hi Rafael,
If it has not been already suggested: try DMSO (20% to 40%).
In my limited experience I found that often DMSO works well for
crystallization conditions with high-salt or high buffer component
(like >1M D,L,-Malic acid).
HTH,
-Partha
On Thu, Dec 17, 2009 at 1:39 PM, Meitian Wang wrot
Please...22 more and I can close out the raffle with the last drawing.
Merry Christmas, BR
-
Bernhard Rupp
001 (925) 209-7429
+43 (676) 571-0536
b...@qedlife.com
bernhardr...@sbcglobal.net
http://www.ruppweb.org/
---
Zhang and Tanner, 2004, Detection of L-lactate in polyethylene glycol
solutions confirms the identity of the active-site ligand in a proline
dehydrogenase structure, Acta Cryst D. 60:985
Dear ccp4 users,
Postdoctoral Fellow Position in Computational Structural Biology at the
Zhang Initiative Research Unit, Advanced Science Institute, RIKEN, Japan
We are seeking a highly motivated and experienced computational
structural biologist to join the Zhang Initiative Research Unit at
I just talked to the NVIDIA pre-sales people and they told me that this driver
(195.22 beta) should work with 120Hz lcd monitors together with Nvidia 3D
vision kit on a quadro graphics card.
Ajit B.
- Original Message -
From: Yong Y Wang
Date: Thursday, December 17, 2009 4:53 pm
Subj
The glasses are not compatible. I tested swapping glasses while keeping
the emitter unchanged and it did not work.
Yong
"David J. Schuller"
Sent by: CCP4 bulletin board
12/17/2009 05:25 PM
Please respond to
schul...@cornell.edu
To
CCP4BB@JISCMAIL.AC.UK
cc
Subject
Re: [ccp4bb] [COOT] 12
On Thu, 2009-12-17 at 13:51 -0800, kai...@caltech.edu wrote:
> On this note,
> I recently bought a viewsonic 120hz LCD (VX2265wm) in the naïve believe it
> should work as it has the right refresh rate. I tried to run it with the
> normal setup, quadro with emitter via DIN, nuVision glasses, Li
Does anyone know if this beta driver
(ftp://download.nvidia.com/XFree86/Linux-x86/195.22) works?
Yong
alexander.schif...@sanofi-aventis.com
Sent by: CCP4 bulletin board
12/17/2009 03:39 PM
Please respond to
alexander.schif...@sanofi-aventis.com
To
CCP4BB@JISCMAIL.AC.UK
cc
Subject
Re: [cc
On this note,
I recently bought a viewsonic 120hz LCD (VX2265wm) in the naïve believe it
should work as it has the right refresh rate. I tried to run it with the normal
setup, quadro with emitter via DIN, nuVision glasses, Linux. Here's the
problem: I have stereo in the top and the bottom but
good point! recently we managed to collect very good room temperature
data with PILATUS detector at SLS. if your crystals are large enough,
say 100 microns or so, you have chance. regards, meitian
On Dec 15, 2009, at 1:42 PM, mjvanraaij wrote:
why not stay with room temp?
many structure
I got the information from a supplier that there is a beta driver from
nvidia that supports the 120Hz LCD with nvidia stereo glasses. I have
not seen it myself but we have a demo scheduled in January.
Alexander
-Original Message-
From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On
Dear CCP4BBers,
Sorry for the non-CCP4 question. I intend to set up some trials for
protein-DNA complexes. In this regard, I have a few queries:
1. After annealing the oligos, how do I ensure that what goes into trials
are only the annealed dsDNA and not other forms such as ones carrying
hairpin
My current understanding is that
1) The new 120Hz LCD monitors are not compatible with the old goggles.
Something about the type of polarization used for the shuttering effect.
So if you were going to try out one of the new 120Hz monitors (~ $400)
you might also want to spend on the nVidia glasses
A postdoctoral position in RNA structural biochemistry is open in my lab at the
Department of Cell and Molecular Biology at the Karolinska Institutet in
Stockholm.
Successful candidates will participate in all steps involved in structure
determination of RNA-protein complexes relevant to tRNA b
Suffering from 'mystery-blob-itis' I searched the BB archives, and
read about the various degradation products of PEG. I used this
information to model my blobs as oxalate. Job done. Except that now I
need a reference for a paper, and I can't find any. Can anyone point
me in the right direc
A postdoctoral research position is immediately available in the
laboratory of Dr. Eric R. Schreiter, at the University of Puerto Rico -
Rio Piedras Campus, to study the effects of S-nitrosylation on protein
structure and function at the molecular level. Applicants should possess
a PhD in a rel
Francois Berenger wrote:
Paul Emsley wrote:
Francois Berenger wrote:
When I run coot in order to read the experimental MTZ corresponding
to the PDB I am viewing, [snip]
I am afraid coot is refining my molecule instead of just displaying
the map.
Well, Refmac is - at Coot's
wwPDB statement on Retraction of UAB PDB entries
-
In its December 4, 2009 issue, the Journal of Biological Chemistry (JBC)
retracted an article by H.M. Krishna Murthy et al. describing the structure of
dengue virus NS3 serine protease published 1
Paul Emsley wrote:
Francois Berenger wrote:
When I run coot in order to read the experimental MTZ corresponding
to the PDB I am viewing, [snip]
I am afraid coot is refining my molecule instead of just displaying
the map.
Well, Refmac is - at Coot's request.
This usually happens because
Francois Berenger wrote:
When I run coot in order to read the experimental MTZ corresponding
to the PDB I am viewing, [snip]
I am afraid coot is refining my molecule instead of just displaying the
map.
Well, Refmac is - at Coot's request.
This usually happens because Coot doesn't detect
Hi Francois,
your mtz file doesnt appear to have any phases, so Coot (or whoever)
cannot make a map. What Coot cleverly tries to do in such a case is to
use calculated phases from the given pdb (and mtz) file. So it runs
refmac with the pdb and mtz to retrieve this phase information. There is
Hello,
When I run coot in order to read the experimental MTZ corresponding
to the PDB I am viewing, I got the following messages (attached is long
lines original log):
---
command: (refmac-for-phases-and-make-map
"/home/berenger/usr/xp_1m6t_14122009/1m6t/1m6t-sf-exp.mtz"
"/DERIV_AU/DERIV_AU/
Jason Porta wrote:
Hi everybody,
I would like to take two mtz files (which are very similar) and calculate
the R-merge between them. I tried looking into CCP4 and Phenix, but could
not find a direct path. Does anybody know how I can do this R-merge calculation?
Best regards,
Jason Porta
If
24 matches
Mail list logo
