I have used phenix.xtriage. It finds three twin laws with alpha ~0.48 for all
of them and the results of the perfect twin test are well over 2.
Ben
On 9/22/09 1:50 PM, Tanner, John J. wrote:
> I suggest you analyze your data with phenix.xtriage. It does several tests
> and gives descriptive
That might be a sign of pseudo-translational symmetry. Check your native
patterson. phenix.xtriage will also tell you. In the presence of
pseudo-translational symmetry, twinning stats will be hard to interpret.
Engin
On 9/22/09 12:31 PM, Ben Flath wrote:
Hi all
when subjecting my data to th
Hi allwhen subjecting my data to the perfect twin test /*2 I get values very close to 3 which is far off the theoretical values of 1.5 and 2 for twinned and untwinned data.can anyone shed some light on what might be going on with my data. Could tetartohedral twinning have anything to do with it?Tha
I think I posted this link maybe two years ago:
http://idb.exst.jaxa.jp/db_data/protein/search-e.php?
Jürgen
On Sep 22, 2009, at 12:48 PM, Elspeth Garman wrote:
or see concentrations given in:
McFerrin and Snell
J.Appl.Cryst (2002) 35, 538
and
Mitchell and Garman,
J.Appl.Cryst. (1996) 29, 584
or see concentrations given in:
McFerrin and Snell
J.Appl.Cryst (2002) 35, 538
and
Mitchell and Garman,
J.Appl.Cryst. (1996) 29, 584
Good luck
EFG
Paul Leonard wrote:
Hi Claudia
It really is best to test the cryo condition in an X-ray beam.
However, if you don't have access to X-rays in hous
Hi Claudia
It really is best to test the cryo condition in an X-ray beam.
However, if you don't have access to X-rays in house you could look at the
hampton crystallization screen conditions (or another supplier). Find a
condition with a similar amount and type of precipitant as you have in
your
Hi,
the Protein-Protein interface analysis server will calculate Gap Volume
and Gap Volume Index:
http://www.bioinformatics.sussex.ac.uk/protorp/
-Konstantin
--
Konstantin Korotkov, Ph.D.
Research Scientist
University of Washington
Department of Biochemistry
Box 3577
It might be risky (and silly), but I have done this successfully. I
just put a small dewar of liquid N2 under the scope, focussed
directly above the surface and looked to see if cryo solutions froze
clear or not. The ones that did worked out when we got to the
beamline (we'd verified that
Hi Claudia
"By eye" is not a bad approximation. The trick I learnt at the JCSG (I
forget the originator, sorry) is to suck up some solution in a P2
pipette tip (the clear ones), dip it in lN2, and then hold it just above
the lN2 (where it's still cold) to check for clearness or not.
Is a go
There is a useful, but out-of-date program in the CCP4 suite called
watertidy.
it analyses the H2Os and after applying symmetry to move them close to
the protein, relabels them according to the protein atom they are closest to
So for protein chain A you can output a water chain W where the
W
Dear Claudia,
please be so kind and tell us all what is / are the crystallization
condition(s)? It would help to us to answer your question!
- J. -
Claudia Scotti wrote:
Dear List,
Sorry for the probably silly question.
Any suggestions to test cryoconditions without X-rays or cryostre
Posted (again) on behalf of PI:
The parasitology group at the Structural Genomics Consortium (SGC) focuses
on structure and function of proteins from apicomplexan and kinetoplastid
parasites, including pathogens responsible for malaria, African Sleeping
Disease and toxoplasmosis. In c
I have not heard of anyone checking for suitable cryo in that fashion how
fast can you do it before it is affected by room temp and how would you know
about the ice rings, but when you have crystal(s) and not crystal you can
freeze different ones in several different cryos (sugars, glycerol etc) an
Hello all,I am trying to calculate the gap volume of a protein-protein complex interface using SURFNET but I cannot locate the download files from: ftp.biochem.ucl.ac.uk . Is there any other program I can try?Thanks.Josiah.
Hi
I've only seen this when people try running Mosflm 7.0.4 (set by the
MOSFLM_EXEC env) with iMosflm 1.0.3. This won't work - there was a bug
that became apparent that could only be fixed by changing both Mosflm
and iMosflm, so if you are running iMosflm 1.0.3, you need to make
sure you
Which compression was used? The packed compression saves a lot of space,
but requires much more CPU involvement. The byte offset compression saves
less space but takes less CPU time. From the numbers, I would guess it
was the packed.
=
Herbe
Waterman, David (DLSLtd,RAL,DIA) wrote:
Bill's example is nice because the compression is transparent,
> so no extra work needs to be done by developers. However, this
> is one for Macs only.
Actually, ZFS is available on Linux too as a user space filesystem, and
Sun are considering a kernel p
Dear List,
Sorry for the probably silly question.
Any suggestions to test cryoconditions without X-rays or cryostream?
I'd need to freeze crystals before going to ESRF and I'm a bit anxious. Is it
enough to try to freeze the cryoconditions in liquid nitrogen checking under
the mi
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