On Monday 06 July 2009, Cale Dakwar wrote:
> Dear colleagues,
>
> Please excuse these naive questions but I am new to the field of structural
> biology and need to ask it.
>
> 1) By submitting my structure to the PDB Validation Server for precheck and
> validation, will my structure become public
Dear
Those bubbles are mostly air - expelled out of the water by heat of mixing
(simple alcohols and water generate fairly huge heat upon mixing).
Crystalline sugars heat of mixing/dissolution is negative (which is why tea
gets cooler when you add sugar to it). Not sure what glycerol+water mixtures
do i
Dear colleagues,
Please excuse these naive questions but I am new to the field of structural
biology and need to ask it.
1) By submitting my structure to the PDB Validation Server for precheck and
validation, will my structure become publicly available in any way?
2)
When I finally submit my str
Perhaps the bubbles are related to the bubbles which form when mixing
alcohols with water, e.g., when making coomassie destain? And what are those
bubbles, by the way?
Jacob
***
Jacob Pearson Keller
Northwestern University
Medical Scientist Training Prog
Hi,
If you're worried - instead of transfering the crystal, just flood the
entire drop with excess of your cryo buffer. No exposure to air this way.
I bet that the bubbles won't go away though :)
Artem
> Dear members,
> I have protein crystals grown in a condition with Isopropanol (13%) and
> ci
Just to add to this post, we've been successfully running Coot on 64 bit
Ubuntu 9.04 using the X86 Centos-5 stable build (0.52) from Paul Emsleys's
site:
http://www.ysbl.york.ac.uk/~emsley/software/binaries/stable/
The only quirk I have found so far is that the window will flicker on and
off whe
Ed Pozharski wrote:
So to answer your question,
there is no decent way of fixing this without compiling coot.
I agree.
What worked for me (eventually) was to build coot using autobuilder
scripts. This is not without caveat, since guile-1.8.5 fails to compile
on Jaunty. I don't know if th
Thanks to all who helped with this, especially Paul Emsley, Ed
Pozharski, Robert Orru, Partha Chakrabarti, and Michael Strickler. Here
is a brief summary of the results:
1. It's easy to compile Coot for 64-bit Ubuntu 9.04 using the autobuild
script *IF* you have all the the prerequisites. S
Have you tried to grow the crystals in ethylene glycol or glycerol instead
of isopropanol?
On Mon, 6 Jul 2009, kumar wrote:
Dear members,
I have protein crystals grown in a condition with Isopropanol (13%) and
citric acid (0.2 M). When I transfer the crystals to cryo-buffer (30%
glycerol or 2
Dear members,
I have protein crystals grown in a condition with Isopropanol (13%) and
citric acid (0.2 M). When I transfer the crystals to cryo-buffer (30%
glycerol or 25% ethyleneglycol with 0.25 M citric acid and 13%-15%
Isopropanol), small bubbles appear on the surface of the crystals. These
ti
> I would like to draw your attention to a protein structure position
> advertised on the AstraZeneca jobs web pages. This is a permanent position in
> a recently formed structural biology team at our site in Boston,
> Massachussets. The position would suit an all-round, hands-on
> crystallogra
Yes, the problem is with 32-vs-64. At first, I have found an ugly
workaround of getting 32-bit version of libguile-srfi-srfi-1-v-3.so.3
and changing the symbolic link in /usr/lib to fool coot into using it.
Coot will start but will be quite unstable. So to answer your question,
there is no decent
I would like to draw your attention to a protein structure position advertised
on the AstraZeneca jobs web pages. This is a permanent position in a recently
formed structural biology team at our site in Boston, Massachussets. The
position would suit an all-round, hands-on crystallographer. The w
AsCA'09 Beijing
Joint Conference of the Asian Crystallographic Association and Chinese
Crystallographic Society
October 22-25, 2009, Beijing, China
Website: http://www.ciccst.org.cn/asca09/
PLEASE NOTE: The deadline for submission of abstracts has been extended to
July 31, 2009.
SPONSORS:
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