Thanks for that, that was in fact the fix. Or else, copy
$CCP4I_TOP/etc/configure.def.dist
to
$CCP4I_TOP/etc/configure.def
and then edit
USE_DBCCP4I_ON_STARTUP
If you don't have write permissions, copy it instead to
~/.CCP4/unix/configure.def
Cheers
ph
Hi.
I noticed long, needle crystals in that very condition (A1 using the
Molecular Dimensions kit) for the protein that I am working with one day.
I tried to optimize that condition not long ago using reagents in my lab.
I did not have a problem with precipitation until using high
concentrations o
Hi All,
there is a condition in the JCSG+ screen (0.2M Li2SO4, 0.1M NaOAc pH 4.5,
50.0% v/v PEG-400) which forms heavy precipitate when made here in the lab.
I assume that the order of addition of compounds might make a difference,
but doesn't this suggest that there is a high probabilty for s
The other use for these ultra-small beams is to illuminate part of a
larger xtal to find the best diffracting (or leat mosaic) regions and/or to
raster out of the radiation damaged areas. This way even "large" xtals
can benefit from this.
Nukri should chime in on this point as well since GMCA-CAT
Yes Colin, of course you are right about the 20-30 micron flight path. I
thought John's question was about micron-size beam, not the micron-size
crystal. Reading it over, I saw my mistake.
As for the micron-size crystals, manipulating those will be another fun
task. Fortunately, tractor beams (whic
Hi
Yes good data with a micron size beam but, in this case, the path length
was 20- 30 micron.
I presume one would like a complete data set rather than a single or a
few processable images. If the latter, then in principle anything is
possible provided background is minimised and a low dose approa
Hi Jon,
You can indeed get data with 1 micron(ish) beam. See for example
http://journals.iucr.org/d/issues/2008/02/00/wd5082/index.html
Different question is whether there is any benefit in using micron size
beam. It is subject of much work and discussion (e.g.
http://www.nsls.bnl.gov/newsroom/eve
Sanishvili, Ruslan wrote:
.. Reasons for discriminating
5-10 micron beams (minibeam) from ca 1 micron (microbeam) might have
been not so much their size but what it involved to achieve these sizes.
Might I ask - do you really get data from 1 micron protein crystals? The
reduction in
Hi,
Is there a way to estimate pI for protein-detergent complexes? Thanks.
Joe
Hi Richard,
Interesting topic raise you did...
On a philosophical level, I would define a microbeam as a beam which
matches in size with a microcrystal. Then question becomes what is a
microcrystal?
If we wanted to be purely scientific, though, we should not be measuring
anything in mm
Just an interesting question of semantics that annoyingly comes up
from time to time when people are comparing x-ray beam diameters.
What counts as "microbeam?"
Of course "micro" has the precise meaning in SI as being a factor of
10^-6.
The problem is that the prefix "micro" just means "extr
Postdoctoral Associate Positions in Structural Biology
Two Postdoctoral Associate positions are immediately available in the
Department of Biochemistry and Molecular Biology at Baylor College of
Medicine. The first position is to focus on structural and functional
studies of influenza virus. The
For other examples of oxidised cysteines you can look at our venerable series
of reverse transcriptase structures... (Stuart, Stammers, Ren, Esnouf and
others) residue 280 on the A chain, from memory.
Regards,
Robert
--
Dr. Robert Esnouf,
University Research Lecturer, Head of Bioinformatics an
I will be out of the office starting 04/20/2009 and will not return until
04/27/2009.
I will be checking email periodically.
Postdoctoral Training Fellow - Structural Biology of Hsp90 Complexes
Section of Structural Biology, ICR
Chester Beatty Laboratories
Chelsea, London, UK
A Wellcome Trust-funded Postdoctoral position is available from 1st
October 2009 in the laboratory of Professor Laurence Pearl FRS, in the
How about an oxidised cysteine? Sulfenic acid is a possibility
(http://en.wikipedia.org/wiki/Sulfenic_acid), although it will
generally oxidise further to sulfonic acid
(http://en.wikipedia.org/wiki/Sulfonic_acid).
I've seen them before in structures of old (4-5 year old) crystals
(see figure 2 o
Hello James,
my first guess would be a second conformation. If the cysteine is part
of a disulfide bridge, it could be a partially broken bridge due to
radiation damage, and the "extra atom" would be the sulfhydryl group
in VdW distance to the former partner cysteine.
Best regards,
Dirk.
Hello All,
I have a couple of cysteines with some extra density about 1 covalent
bond's length away from the sulfur center. It looks to be one atom's
worth of extra density. Because I could fit it in an icon sized
graphic and I anticipate that someone will suggest I post a picture,
I'm at
Try $CCP4/ccp4i/etc/unix/configure.def
Given your panic, am aiming for quick rather than complete answer ...
(sorry, was too busy to answer at 3am).
m
On Tue, 2009-04-21 at 03:44 +0100, Frank Von Delft wrote:
> Yes, I was framed. Thanks for the quick response.
>
> Okay, question 2:
> There is
Coot will read CNS maps. Is the CNS mask format different to the CNS map
format? If so, convert your CNS mask to a CNS map first. (I presume CNS
can do this - I've not used it.)
Xiaowei Pan wrote:
>
> hello Crystallographers
>
> I have generated a solvent mask by CNS ,how can I open the .mask f
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