Hello James,

my first guess would be a second conformation. If the cysteine is part of a disulfide bridge, it could be a partially broken bridge due to radiation damage, and the "extra atom" would be the sulfhydryl group in VdW distance to the former partner cysteine.

Best regards,

Dirk.

Am 21.04.2009 um 11:39 schrieb James Stroud:

Hello All,

I have a couple of cysteines with some extra density about 1 covalent bond's length away from the sulfur center. It looks to be one atom's worth of extra density. Because I could fit it in an icon sized graphic and I anticipate that someone will suggest I post a picture, I'm attaching a picture of the positive fofc density.

Does anyone have any idea of the usual culprits here? I see no negative density in the region.

James

<greencys.png>


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Dirk Kostrewa
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