Re: [ccp4bb] removal of dimer

Thanks a lot for all your suggestions. But how much DTT or BME shall i add. Also i fear that these will interfere in the formulation and how shall i get rid of them then. As for GPC my protein is 19KDa and diemr will be approx 38 KDa and it is difficult to separate them without losing the protein

Re: [ccp4bb] removal of dimer

Why don't you add some reducing agent like DTT or BME to make everything as monomer? Anthony On Tue, 17 Feb 2009 10:47:28 +, Meg wrote > I have purified my protein granulocyte colony stimulating factor using > chromatography steps. my protein is relatively pure when analysed by > reducing

Re: [ccp4bb] expression of single chain variable fragment

Both will probably work. Expression in insect cells is most likely to work well, but will of course be more expensive and time consuming. There's also some slight possibility of getting unwanted glycosylation - which is not necessarily a problem. I've had good success expressing VHH fragments (s

Re: [ccp4bb] reifine metal with phenix

Hi Lisa, in addition to what Joern suggested, there are two other options: 1) You can run "phenix.metal_coordination" that will create additional restraints for Mg atoms: phenix.metal_coordination model.pdb 2) Not the best ways of doing this, but just to mention so you have a complete list

[ccp4bb] expression of single chain variable fragment

Dear all, I'd like to express a single chain variable fragment (scFv) for crystallization purposes, but cannot decide whether I should do it in insect cells (let the antibody be secreted into culture medium) or bacteria (let the antibody be secreted into periplasmic space). If someone could give

[ccp4bb] PyMOL: How to relax geometry constraints for cartoon mode/

I think this was posted earlier, but the geometry constraints for the cartoon mode is a little tight for a residue of my protein. What's the setting to relax them so it'll draw a continuous tube instead of leaving it blank? Thanks FR - Francis R

[ccp4bb] Postdoctoral position at EMBL Grenoble

*Postdoctoral fellowship at the High Throughput Crystallization laboratory of the EMBL Grenoble Outstation* *EMBL site:* EMBL Grenoble *Commencing date:* As soon as possible *Job description:* The High Throughput Crystallization laboratory at the EMBL Grenoble Outstation is offering a posdo

Re: [ccp4bb] screensaver [ or better yet, an automatically generated RSS feed]

Jürgen In the case you have an overwhelming amount of images, why not instead just setup an automatically generated RSS feed (on a server that you or maybe the ccp4 project or wiki will host) that contains them? There is an RSS screensaver already built into leopard, I'm sure there is on

[ccp4bb] screensaver

Hi all, let me see your pictures. I'll prepare a CCP4BB Screensaver for Win(we'll see) & Mac (guaranteed). Each individual may send two of his/her best pictures related to crystallography, you must have either a) the permission to distribute this picture or b) be the owner of it (keep in mind if

[ccp4bb] removal of dimer

I have purified my protein granulocyte colony stimulating factor using chromatography steps. my protein is relatively pure when analysed by reducing and non-reducing SDS PAGE method. however non-reducing page shows DIMER presence. i have about 250 ml pure protein sample and do not want to perfo

Re: [ccp4bb] Refinement

More details? Are there any particular data problems ? Is twinning a possibility - look at new truncate plots.. Eleanor Rana Refaey wrote: Hi all I have two datasets of resolutions 1.6 and 1.65 Å both of the same molecule, the problem that i am facing is the refinement. The R factors are stu

Re: [ccp4bb] PDB protein strucutrues as screen saver

If you choose one of the structures we solved for your T-shirt (admittedly not as sexy as rna polymerase or hiv protease or 1aka), don't worry about acknowledging us. What's more, if we ever meet you wearing the T-shirt at a conference or somesuch, you'll be guaranteed a free drink! Mark Ma

Re: [ccp4bb] unstable refinement

You dont say at which point in the refinement cycle you are.. The refinement algorithms are meant to reduce these parameters, and if they diverge wildly there is probably something seriously wrong either with the software or the model.. eg - different cell dimensions or space group for the coor

Re: [ccp4bb] PDB protein strucutrues as screen saver

Hi everybody, Thanks for the information, Even i was discussing with one my uncle who is in fashion industry,about these intelligent designs (offcourse the pbd structures). May be folks..in future you have to wear rna polymerase or hiv protease or 1aka or. imprinted on holiday shirts..is that