[ccp4bb] Published derivation of mFo-DFc formula?

2008-12-30 Thread Ian Tickle
All, something for you to think about over the Study Weekend - sorry I can't be there with you this time :-( : I was recently asked whether a formal derivation of the expression we use for the 'minimally biased' difference Fourier coefficient, i.e. delta-Fm = mFo-DFc, is published anywhere, and I

[ccp4bb] organic Cl as H-bond acceptor?

2008-12-30 Thread Edward A. Berry
Dear all, Is it possible for a Cl atom attached to an aromatic ring to accept an H-bond from His NE2? I'm modeling a low-resolution ligand structure and my favorite fit shows such a bond, but it is possible the ring is flipped and an OH or -C=O on the other side of the ring actually make the H-bon

[ccp4bb] summary: crystals from skin

2008-12-30 Thread riya doreen
Hello Everyone, Thank you for all the suggestions on how to manipulate skin on crystal drops. Here is a summary of responses: (i) Higher concentrations of reducing agents (ii) Layering crystal drop with cryo-protectant (iii) Trypsinize the skin (iv) Grow crystals under oil (v) Try 4C if crystals

Re: [ccp4bb] MR- Problem-76 % seqeunce identity-no solution

2008-12-30 Thread Meetmr Ss
Dear all and specially Eleanor, Thank each and everyone who responded to my query. All your suggestions made me to think in all possible dimensions. I have good news to share with all of you. Balbes gave me marginal solution with model which had 29% sequence identity. At present I am doing

Re: [ccp4bb] MR- Problem-76 % seqeunce identity-no solution

2008-12-30 Thread Eleanor Dodson
Meetmr Ss wrote: Dear all, I struck with MR problem. MY target has 76% sequence identity with the model. I tried Phaser, AMoRe and Molrep. None of them gave me satisfactory solution. If you have any suggestions and or New programs I would like to try. Thanks somu Is your space g