Hello Everyone, Thank you for all the suggestions on how to manipulate skin on crystal drops. Here is a summary of responses:
(i) Higher concentrations of reducing agents (ii) Layering crystal drop with cryo-protectant (iii) Trypsinize the skin (iv) Grow crystals under oil (v) Try 4C if crystals are grown at 18 or RT (vi) Lower protein concentration and (vii) Fiddling around with the drop which may break the skin and free the crystals On Sun, Dec 28, 2008 at 8:24 PM, riya doreen <driy...@gmail.com> wrote: > *Hello All, > > I am trying to optimize protein crystals that grow from skin. All efforts > to separate crystals from skin have failed since it is fairly thick. > Crystallization under oil eliminates skin but the resultant crystals are > very thin and small needles. > > I would appreciate any suggestions on how this problem can be eliminated. > > Thanks > > *
