MAIN does that for ages (n_molecules->generate_others). Also, the new COOT can
do that (look in the - rather cluttered - treasure
chest 'Extensions'->'NCS'->'Copy NCS chain')
HTH
-j.
On Wednesday 26 November 2008 00:25:12 Prakash Rucktooa wrote:
> Dear Olga,
>
> I have also encountered the sam
I switched to mineral oil (same as for pcr). You can apply it either with a
1mL pipet tip or with use a glass vial with the same diameter as the linbro
wells as a stamp. This is much nicer as round cover slips do self-center and
opening is gentler on the slips, too.
HTH,
Jens
On Thursday 27 N
On Friday 28 November 2008, Donnie Berkholz wrote:
> On 16:04 Fri 28 Nov , Ethan A Merritt wrote:
> > On Friday 28 November 2008, Ben Akiyama wrote:
> > > I am measuring and comparing torsion angles in several solved
> > > crystal structures of RNA helices using AMIGOS. I was wondering if
> >
On 16:04 Fri 28 Nov , Ethan A Merritt wrote:
> On Friday 28 November 2008, Ben Akiyama wrote:
> > I am measuring and comparing torsion angles in several solved
> > crystal structures of RNA helices using AMIGOS. I was wondering if
> > anyone knows of a program that can give me an idea of the
On Friday 28 November 2008, Ben Akiyama wrote:
> Hi Everyone,
>
> I am measuring and comparing torsion angles in several solved crystal
> structures of RNA helices using AMIGOS. I was wondering if anyone knows
> of a program that can give me an idea of the error in these measurements
> based on
Hi Everyone,
I am measuring and comparing torsion angles in several solved crystal
structures of RNA helices using AMIGOS. I was wondering if anyone knows
of a program that can give me an idea of the error in these measurements
based on coordinate error, b-factors, etc.
Thanks,
Ben Akiyama
On Friday 28 November 2008, Mueller, Juergen-Joachim wrote:
>
> Dear all,
> does anybody know a program to
> fill an unit cell a,b,c randomly by an arbitrary number
> of spheres (atoms)?
First you would need to define "random".
Uniform density throughout the lattice?
Uniform distribution of nei
Dear all,
does anybody know a program to
fill an unit cell a,b,c randomly by an arbitrary number
of spheres (atoms)?
Thank you for help,
Juergen
Hello All!
Does anyone know if is there available in the market some sort of
all-in-one device capable of keeping the crystals of a double crystal
monochromator tunned?
I've contacted colleagues around the globe who have similar systems
running, but none of them were able to indicate me some co
Well, we were gearing up for a release tomorrow, but a major problem
with mmdb has come to light. We are moving to fix this and get
binaries on the ftp site. So please hold off until it is announced.
Charles Ballard
CCP4
We are seeking an experienced protein crystallographer
interested in the study of cell and virus glycoproteins to join our group
at the Centro Nacional de Biotecnología (Spanish Research Council)
Madrid, Spain
(
http://150.244.80.11/content/research/macromolecular/interactions/index.php?l=0).
Ap
Very long soaking time will also help.
In your question why the binding kinetics was so slow
Here are some explanations
Diffusion Rate depends from solution viscosity, size of the molecule that
moves in this solution and the pores it has to go through.
The binding kinetics in the crystals are usua
Dear Priya,
How long did you soak your crystals?
I had a similar problem. When I tried to co-crystallize my protein with
its substrate (5 mM) I never got any substrate bound. I tried soaking
with higher concentration (up to 50 mM) and this didn't work either. The
crystals were infinitely stab
Their presence there signifies that we are in the final stages of
testing the downloads However we are still finding problems, hence
no announcement.
If you take from here, most things will work most of the time. However,
they are definitely not supported, and we won't take time out from
prep
Dear all,
does anybody know a program to
fill an unit cell a,b,c randomly by an arbitrary number
of spheres (atoms)?
Thank you for help,
Juergen
What is the solubility of substrate?
Try analogues with higher solubility. The 2mM may not be 2mM in the
solution!
The fact that you added there does not mean that it goes to the solution.
If solubility is indeed high and Ki/[solubility]>1 you should be able to see
some density in the binding s
hi there all
just a final reminder about the BSG BCA winter meeting
on tuesday 16th december. i need to finalise numbers
with the caterers soon, so please can you register,
(or failing that just send me an email), by friday 5th
december. many thanks.
http://conferences.ncl.ac.uk/BCA_BSG_Winter_2
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