Hi,
Yes. This is very similar to what we've observed previously - and the answer
in those earlier cases was - oligomerization. This is doubly likely since
you have SEC that suggests this possibility as well.
An additional consideration is that your ladder follows (at least by
eyeballing) an almo
Hi,
Though I do not claim to be a data guru (like some of the true wizards of
crystallographic theory who post here) - I would suggest that the
'improvement' in the Rfree does not in any way represent a better fit of the
model to the data but rather is a consequence of the fact that our new Rfr
Hi,
I have been working on the 2A data. Though almost done, R/Rfree was
around 0.20/0.27. Then I noticed that structural factors were
reindexed from P222 to P212121 after scaling/merging in the early
process (I am finishing up a little bit old data). To escape any
problems from wrong merg
Thanks to all who answered (so quickly). To summarize, the premise
fails because NMR/FRET and crystallography are based on different
phenomena, and the energy-distance relationship is only valid for the
latter. One has to wonder why they need to build bigger and bigger
accelerators instead of inv
This is related to the "atomic resolution in NMR" question discussed
earlier. I have encountered a paradox and I suspect that someone in the
bb might have an answer, so help me not to invent the wheel. Also, if
you strongly believe that ccp4bb is only to be used for CCP4(or
crystallography)-relat
Somebody asked about cysteines:
only one cysteine (therefore only dimers possible), but the native gels are
exactly the same with lots of DTT anyway.
JPK
***
Jacob Pearson Keller
Northwestern University
Medical Scientist Training Program
Dallos Laborato
Jacob - To me it looks like your data are inconsistent. I would first
clear up the apparent discrepancy between your AUC and SEC data.
Regarding the native gel, you seem to have a mixture of fairly stable
species that don't interconvert much. If mass spec gives you only one
species, I would
Dear Crystallographers,
I am having trouble interpreting the attached native gel (which is very
repeatable, under various conditions). Is it tenable that various charge
states or conformations could account for this behavior, or must it be
various oligomeric states? AUC results seem to show on
I know that this has been posted before, but I have lost track of the
tread. I would like some nice SeMet lysozyme data for our
crystallography class for graduate students at UNC. Does anyone know
where I can find those data to download?
thanks,
-ed collins
Edward J. Collins
Associate Pr
Thanks Eleanor and everyone else that replied privately.
Twinning was a common thought and the data was not by several tests.
The MR was solved by getting better data, clean 2.8A vs. the 3.0A I had.
With this data phaser was able to solve it easily in R32 with 3
molecules. There was not signifi
Thanks. The preferences have already been set. Unfortunately, steps
1-4 did not work for me.
Finally, here it is:
Shift+Ctrl+two-finger-scrolling on trackpad did it!
Thanks,
Raji
On Nov 19, 2008, at 12:58 PM, Jack Tanner wrote:
This works on a Mac laptop running 10.5.5.
Go into system p
... and once you've got the hang of that, go out and buy yourself a game of
Twister ...
-Original Message-
From: CCP4 bulletin board on behalf of Jack Tanner
Sent: Wed 19/11/2008 9:58 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Pymol: Zoom without Mouse
This works on a Mac lapto
This works on a Mac laptop running 10.5.5.
Go into system preferences for keyboard and Mouse. Select the trackpad
menu. Click the boxes for "use two fingers to scroll" and "allow horizontal
scrolling".
Then, in pymol do this:
1. Place middle and ring finger on trackpad.
2. With the two finge
move z, [number] (where [number] is (of course) a number) will do it but you
have to be careful about clipping. There may well also be a zoom command ...
-Original Message-
From: CCP4 bulletin board on behalf of Raji Edayathumangalam
Sent: Wed 19/11/2008 9:29 AM
To: CCP4BB@JISCMAIL.AC.U
How does one zoom into the molecule in Pymol without a mouse and with
just the Mac trackpad and keyboard?
Have tried to look it up in the manual and on the web. No success
finding it yet; I did figure it out once before but can't redo it now
for the life of me. Need to know how to do it wit
Axel:
Um... it works fine for us. I just tested it about 10 seconds ago
(names have been changed to protect the guilty):
[EMAIL PROTECTED]> ssh [EMAIL PROTECTED]
Password:
macclient:~ myaccount$ pwd
/Network/Servers/osxserverbox.some.domain/afp/myaccount
macclient:~ myaccount$ echo $HOME
/Networ
On Nov 19, 2008, at 12:15 AM, Harry M. Greenblatt wrote:
There are, of course, alternatives these days in the Unix world to
NFS, in the form of other distributed file systems,
Let us not forget the most sane and far superior alternative to a
distributed file system: a local file system. I'v
BS"D
Our experience with an Xserve-XRAID (10.3.x) as an NFS file server
for a mix of Linux and Mac clients was "less than satisfactory", to
put it politely. In fact, we gave back the Xserve and RAID for a
full refund (that was a precondition of purchase), and got a Linux
file server.
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