Re: [ccp4bb] Substrate(water insoluble) Soaking

Hi there... http://www.ncbi.nlm.nih.gov/pubmed/17164529 Hassell, AM et al, (2007) Crystallisation of Protein-ligand complexes Acta Cryst D63,p72 This paper is an excellent review of "how to get my ligand into my crystal" and includes some methods for dealing with insoluble ligands. HTH, David

[ccp4bb] Substrate(water insoluble) Soaking

Hi All, I am now soaking two substrates to the crystal.But one is soluble in ethanol and one is soluble in DMSO. After co-crystallization and soaking, no substrate found in the structure. Does anybody has the experience in dealing this problem? Any comments or suggestions wo

Re: [ccp4bb] foam dewar usage ?

Hi everyone, Apologies for any confusion my previous posting may have caused. Here are a few clarifications: - The development of the dewars was coincident with a transition in the BCSB management from Thomas Earnest to Gerry McDermott and John Kuriyan. - Gerry McDermott confirmed that the

Re: [ccp4bb] Potential N-terminal cyclization - 2nd attempt with formula attached

Hi, Not sure what you mean by 'other way around' - the formula you drew is what I was thinking about :) Mesomeric states in normal amidines are averaged and should result in planarity - since this case does not seem to have a planar geometry, there's a chance that the amidine never formed and the

Re: [ccp4bb] Potential N-terminal cyclization cont.

Thank you. I was thinking along the lines of amination but was concerned about the reactivity of the His nitrogen... To add more information about the system: this is an untagged thermophile protein, no His column used for purification. It was heated to 65 degC during purification at pH 6.8 a

Re: [ccp4bb] foam dewar usage ?

We are talking about the same containers. Actually, Jon Spear contacted me today and indicated that the burrs are probably left-overs from the milling process which were not cleaned out sufficiently. This explains the particles and the vigorous boiling action caused by the abundance of nuclei fo

[ccp4bb] A copy of Nucplot please

Sorry. That should have read 'a copy of Nucplot' and not nuvplot. - Original Message - From: Raymond Yengo <[EMAIL PROTECTED]> Date: Friday, October 10, 2008 2:53 pm Subject: [ccp4bb] A copy of Nuvplot please! > Hi all, > I have been trying to download a copy of nucplot but the links >

[ccp4bb] A copy of Nuvplot please!

Hi all, I have been trying to download a copy of nucplot but the links sem to be dead. The authors have not replied to my numerous mails either. Can some one out there help me out? Raymond

Re: [ccp4bb] Potential N-terminal cyclization - 2nd attempt with formula attached

Dear Colleagues, The amidine originating from the condensation reaction between the Met N-formyl group and the His side chain should be the other way around! This would also be roughly consistent with the reported geometry. In fact, there should be two mesomeric states, provided there is N pr

Re: [ccp4bb] Potential N-terminal cyclization

Dear Colleagues, The amidine originating from the condensation reaction between the Met N-formyl group and the His side chain should be the other way around! This would also be roughly consistent with the reported geometry. I hope you can see the formula: Arne Skerra At 5:56 Uhr -0400