This sounds to me like a compiler problem on your machine. Try using
the latest Intel Ifort, Portland Group pgf95, or GFORTRAN compiler.
Axel Brunger
On Sep 12, 2008, at 2:56 AM, jxqi wrote:
Dear ccp4bbs,
When I was refining a protein structure using the rigid.inp in
CNS(Version 1.21), an
Hello Matt,
did you use a nickel (or similar) affinity column in the purification + is
there any beta-mercaptoethanol or DTT in your sample? Nickel can easily
'bleed' off the column and will turn brown when complexed with bME or DTT -
this complex can get quite large and even insoluble if enough
Hi Matt,
I sometimes see a similar thing with my proteins, which definitely don't
possess metal co-factors or prosthetic groups. I found that gel filtration
got rid of it - the browny-yellow stuff came out in the void fraction so I
figured it was aggregated protein. I think it was aggregation via t
Dear ccp4bbs,
When I was refining a protein structure using the rigid.inp in CNS(Version
1.21), an error occured saying that the asymmetric map unit is incompatible
with the symmetry operator.The model I used was first refined by the Rafmac5.I
had met the same problem when I tried to add water
