Dear Lucas
It looks to me that the angle of the VCM and therefore of the reflected pink
beam which has double the angular change may be your major contributor. This
could be coupled and complicated by any vertical movement in the source. Things
I would look at are the motors on the kinematic j
You can solibilize IB with your solublization buffer, measure the
solubilized protein concentration by a protein assay like Bradford. Then
adjust the concentration.
Most weight of IBs is not from proteins. However, your protein should be the
predominant protein in the IB if the expression is done
We have our recombinant protein expressed in inclusion bodies we just wash
the IBs and perform solubilization and refolding. Our solubilisation protocol
mentions making up the volume to contain 7-8 mg / ml protein.
1. how can we determine the total protein content in the inclusion bodies
itself
Hi,
I assume that there is no other way and you have to share the space, so I
will save you from the standard tirade about functional space segregation
:-)
If the air in the lab is so loaded with solvents that your plates are
melting - then *you* (or anyone else for that matter) certainly s
If resolution is around 2.0 or better, ARP/wARP is particularly
powerful for such cases when model bias needs to be reduced.
For more info:
http://www.ncbi.nlm.nih.gov/pubmed/18094467?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum
http://www.ncbi.nlm.nih.g
Dear all,
Sorry for an extremely off-topic question, but I thought this would be the best
place to find an answer.
We are setting up our protein crystallography lab and have been asked to share
laboratory space with an organic chemistry group which is already well
established in the room. Th
Hi Sampath,
On Sat, Jul 26, 2008 at 02:05:17AM +0900, Sampath Natarajan wrote:
> Also I could find many cuts in the density.
This looks to me like a problem with your low-resolution data? Since
you collected 1.6A data my guess is that you probably had a fair
amount of overloads. Did you do a low-
Hello Michele! Thanks for your answer!...
Answering your questions we have a collimating mirror upstream the mono
and the water temperature used to keep this guy cooled is within
+/-0.3°C. Regarding fluctuation in the water flow, I dont know, maybe
not cause we have a dedicated thermal bath wit
Hi there,
Following the instructions I got from the mail list (thanks to them) I
ran sfchek to get the rscc. When I did it I could nor find the exact
term of rscc but there is a "Correlation Factor" into the Model vs.
Structure Factor chart. Is that "Correlation Factor" the rscc I am
looking
Dear all,
Thank you so much for the good responses. I will try to get good model again
with your suggessions. If I have problem again I will let you know.
Thank you very much again for your suggessions who are all responded me.
With Regards,
Sampath
Hi again,
I am trying to calculate the rscc of the model to the electron density
map. Any help will be appreciated.
Thanks,
Ariel
--
Ariel Talavera, Lic.
Dept. of Computational and Structural Biology
Center of Molecular Immunology
P.O.Box 16040, Havana 11600
Cuba
tel: (53-7) 271 7933, ext. 21
Hi every one,
Please, I need help to calculate the rcss.
Thnaks in advanced,
Ariel
--
Ariel Talavera, Lic.
Dept. of Computational and Structural Biology
Center of Molecular Immunology
P.O.Box 16040, Havana 11600
Cuba
tel: (53-7) 271 7933, ext. 219
fax: (53-7) 272 0644
email: [EMAIL PROTECTED]
Hi Bill,
I commented out the export DISPLAY lines, but it didn't help. What did fix
it was reinstalling x11 from the OSX 10.5 CD. Any ideas? I've attached the
launch script as a text file.
Thanks!
Kendall
On 7/25/08 12:27 PM, "William G. Scott" <[EMAIL PROTECTED]> wrote:
> Hi Kendall:
>
> I h
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