Hi Sampath, On Sat, Jul 26, 2008 at 02:05:17AM +0900, Sampath Natarajan wrote: > Also I could find many cuts in the density.
This looks to me like a problem with your low-resolution data? Since you collected 1.6A data my guess is that you probably had a fair amount of overloads. Did you do a low-resolution pass? What is the completeness in the 20-8A range? If there are problems with the low resolution (e.g. lots of very strong reflections missing due to overloads or a large and not well masked beamstop) then it can easily happen that you can't find the heavy atom sites and/or the molecular replacement solution isn't very good. The few low resolution reflections are much more important for the intial structure solution (no matter if experimental phasing or MR) than those thousands of high-resolution reflections ... If you see nice density for a helix but with lot's of gaps (that don't make sense) this could be one of the reasons. Cheers Clemens -- *************************************************************** * Clemens Vonrhein, Ph.D. vonrhein AT GlobalPhasing DOT com * * Global Phasing Ltd. * Sheraton House, Castle Park * Cambridge CB3 0AX, UK *-------------------------------------------------------------- * BUSTER Development Group (http://www.globalphasing.com) ***************************************************************
