Hi there,
Sorry for the off topic questions. We need your feedback.
We are expressing a rat protein in insect cells. It is expressed as a secreted
protein with an N-terminal 6xHis tag. We can get about 4 mg of it from 1L
culture and everything looked quite normal at the very beginning (at 4C)
Thermolysin binds a catalytic zinc and several Ca ions for structural
stability. See Holland et al. (1995) Protein Sci, 4, 1955.
Hope that helps,
Arthur Glasfeld
Department of Chemistry
Reed College
3203 SE Woodstock Blvd.
Portland, OR 97202
USA
On Jun 12, 2008, at 8:15 PM, Neeraj Kapoor wr
I think S100 binds both Zn and Ca.
On Thu, Jun 12, 2008 at 11:15 PM, Neeraj Kapoor <
[EMAIL PROTECTED]> wrote:
> Hi all,
> I recently came across a question about an interesting idea. Does
> anyone know of an example of a protein binding to both Zn2+ and Ca2+ at the
> same time? Are there a
On Thursday 12 June 2008 20:15, Neeraj Kapoor wrote:
> Hi all,
> I recently came across a question about an interesting idea.
> Does anyone know of an example of a protein binding to both Zn2+ and
> Ca2+ at the same time? Are there any known well studied precedents at
> all if any. Any h
Hi all,
I recently came across a question about an interesting idea.
Does anyone know of an example of a protein binding to both Zn2+ and
Ca2+ at the same time? Are there any known well studied precedents at
all if any. Any help of insights would be very valuable.
thanks,
Neeraj
But it seems that Hendrik Lorentz was the first to realise that symmetry
breaking of the isotropy of the refractive index & other optical properties
could occur in cubic crystals at sufficiently short wavelength even in the
absence of a distorting force - the "spatial-dispersion-induced birefrin
I would like to also add that depending on what you want to
use the test data for, you may find that the data JCSG has deposited
with the PDB is sufficient.
The JCSG crystal structures include the following data sections in
the structure factor file deposited with the PDB (since spring 2004).
1
Hi all !!
I am working with two data sets of same protein (130a/a) with resolutions
2.8 and 3.2 A. In both the cases the density for 100-130 aa is not very
clear.. it forms couple of helices.. i can see a long tube going but it is
feature less.. It is a MR solution.. i have tried TLS refinement
On Thursday 12 June 2008 11:19, Philippe DUMAS wrote:
> Hello,
>
> A short comment of historical interest: the first theory about "double
> refraction in crystals" (with explicit calculation of the index ellipsoid)
> goes back to 3 memoirs by A. Fresnel in 1821 and 1822. So, we are even in
> "olde
Hello,
A short comment of historical interest: the first theory about "double
refraction in crystals" (with explicit calculation of the index ellipsoid)
goes back to 3 memoirs by A. Fresnel in 1821 and 1822. So, we are even in
"older regions".
This being said, in cubic crystals the index ellipsoi
PS in case you missed it, here's the bottom line from the paper:
"Interestingly, a cubic crystal has seven nonbirefringent axes, four in
the <111>
directions and three in the <100> directions, with birefringence maxima
in the twelve <110> directions."
So it would appear that the optical propertie
All,
Thanks to everyone who has recently requested access to the
JCSG dataset archive. We have a large and growing number of users.
Your requests will be handled shortly and you will receive
notification via e-mail.
Our registered users fall into 3 main classes:
1) Methods developers who want
Hi Ethan
You could be right, see this paper:
http://physics.nist.gov/Divisions/Div842/Gp2/DUVMatChar/PDF/IntBiref.pdf
Cheers
-- Ian
> -Original Message-
> From: [EMAIL PROTECTED]
> [mailto:[EMAIL PROTECTED] On Behalf Of Ethan A Merritt
> Sent: 12 June 2008 15:46
> To: Multiple recip
A post-doctoral position and a technician position available in the
Netherlands Cancer Institute in the groups of Titia Sixma. The
Netherlands Cancer Institute is a center of excellence with a high
standard of biological research and an interactive atmosphere. It is
located in Amsterdam, with all
Let us not forget about the very fact that X-ray is an ionizing radiation and a
potent creator of radicals! Please see these references - seeing a crystal that
was purple in the middle (0.3mm radius of beam) and perfectly yellow at edges
(crystal was >0.5mm long) was an eye opening experience. H
Hi Subbu,
Here's my two cents.
Your project sounds really interesting.
You mentioned in your email that the active site and associated waters
in the active site of the wild type and mutant enzymes are
"identical". It's worth noting that even very small (on the 1/10th of
an angstrom level)
On a general note, it is not unusual at all for a random mutation (i.e.,
one not in the active or regulatory site of an enzyme, and not
significantly connected with the catalytic or regulatory mechanism) to
affect the rate constant of an enzyme-catalyzed reaction (kcat or
kcat/Km) by a factor o
On Wednesday 11 June 2008 23:55, Robin Owen wrote:
> Hi Jacob,
>
> The birefringence of a crystal is determined by a three dimensional
> shape (the indicatrix) describing how refractive index varies with
> direction within the crystal. You can think of this as a 3d ellipse and
> the birefringen
I assume you are talking about a sugar-binding enzyme ;) I have some
aspects to consider in addition to what Artem raises. Many effects of
a mutation are not recognizable in a static crystal structure or even
in an NMR structure. For example, it is usually difficult to assess
the thermodyna
Jayashankar schrieb:
Dear scientists and friends,
I am much interested to learn things by my own.
There are many guys out there as me.
To fulfill the thirst and quest for more crystallographic practical
knowledge, I need test data set for
SAD,MAD,SIR,MIR,SIRAS,MIRAS.
And I want to remind kev
The crank/dm/buccaneer tutorial is now available here:
http://www.ysbl.york.ac.uk/~cowtan/buccaneer/tutorial/tutorial.html
More data is available in your $CEXAM directory. There are datasets for
RNAse and toxd dataset - both SIR/MIR. In the CVS version there is also
SIRAS data for rnase and a
Hi Neeraj,
Try pdbsum. looks nice for mine. You can upload your structure there...
http://www.ebi.ac.uk/thornton-srv/databases/pdbsum/upload.html
hth...
gregor
-Ursprüngliche Nachricht-
Von: CCP4 bulletin board [mailto:[EMAIL PROTECTED] Im Auftrag von
Neeraj
Gesendet: Mittwoch, 11. Ju
Hi
We have just launched TARDIS - a federated repository for raw
diffraction data: http://www.tardis.edu.au/
So far it contains MR/MIRAS data, but will expand soon
cheers
Ashley
On 12/06/2008, at 10:40 PM, Jayashankar wrote:
Dear scientists and friends,
I am much interested to learn thing
Hi,
For MR/SAD/MAD data, the JCSG have made most of their data available -
the processed data at a number of stages (integrated, scaled etc.) and
quite a lot of the raw diffraction data. If you google "jcsg" they are
the first hit. If you look at the structure gallery you can download all
the fil
Dear scientists and friends,
I am much interested to learn things by my own.
There are many guys out there as me.
To fulfill the thirst and quest for more crystallographic practical
knowledge, I need test data set for
SAD,MAD,SIR,MIR,SIRAS,MIRAS.
And I want to remind kevin and Martyn that they t
Hi Jacob,
Chek out Section 2 in the following paper:
Echalier et al. (2004) Assessing crystallization droplets uding
birefringence.
Acta Cryst D60, 696-702.
It offers a very effective summary of the physical basis of crystal
birefringence and reiterates the classification of crystal optics based
Hi Jacob,
The birefringence of a crystal is determined by a three dimensional
shape (the indicatrix) describing how refractive index varies with
direction within the crystal. You can think of this as a 3d ellipse and
the birefringence is given by the difference in length of the two axes
of th
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